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Host-pathogen interactions. XIII. Extracellular invertases secreted by three races of a plant pathogen are glycoproteins which possess different carbohydrate structures

Journal Article · · Plant Physiol.; (United States)
DOI:https://doi.org/10.1104/pp.59.6.1104· OSTI ID:7218594
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The invertase present in the culture fluid of races 1, 2, and 3 of Phytophthora megasperma Drechs. var. sojae A. A. Hildebrand (Pms) were purified until they gave but a single band, whether stained for protein or carbohydrate, after isoelectric focusing in flat bed gels. The sugar compositions of multiple preparations of the purified invertases from each race of this fungal pathogen were determined by quantitative gas chromatography of their alditol acetates. The invertases are composed of about 25 percent carbohydrate. Mannose and glucosamine make up more than 97 percent of the carbohydrate portions of the invertases of all three Pms races analyzed, but the ratio of mannose to glucosamine is clearly not the same in each race. The glycosyl linkage compositions of the glucosamine-containing mannans of multiple preparations of the Pms invertases were determined by GC-MS analysis of the partially methylated alditol acetate derivatives. The results of these analyses demonstrate clear quantitative differences between the glycosyl components of the different Pms races. The existence of race-specific carbohydrate structures in the differentially virulent Pms races suggests that these carbohydrates may be involved in determining the specificity of host-pathogen interactions.
Research Organization:
Univ. of Colorado, Boulder
OSTI ID:
7218594
Journal Information:
Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 59:6; ISSN PLPHA
Country of Publication:
United States
Language:
English

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Related Subjects

550200* -- Biochemistry
550900 -- Pathology
59 BASIC BIOLOGICAL SCIENCES
ACETATES
ALDEHYDES
AMINES
BACTERIA
BIOCHEMICAL REACTION KINETICS
BIOMASS
CARBOHYDRATES
CARBOXYLIC ACID SALTS
CHROMATOGRAPHY
ENERGY SOURCES
ENZYMES
FUNGI
GAS CHROMATOGRAPHY
GLUCOSAMINE
HEXOSAMINES
HEXOSES
HOST
KINETICS
MANNOSE
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PATHOGENESIS
PLANTS
PROTEINS
REACTION KINETICS
RENEWABLE ENERGY SOURCES
SACCHARIDES
SEPARATION PROCESSES