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Sensitive assay of GTP cyclohydrolase I activity in rat and human tissues using radioimmunoassay of neopterin

Journal Article · · Anal. Biochem.; (United States)
; ; ; ; ; ;

A highly sensitive and simple assay for the activity of GTP cyclohydrolase I (EC 3.5.4.16) was established using a newly developed radioimmunoassay. D-erythro-7,8-Dihydroneopterin triphosphate formed from GTP by GTP cyclohydrolase I was oxidized by iodine and dephosphorylated by alkaline phosphatase to D-erythro-neopterin, and quantified by a radioimmunoassay for D-erythro-neopterin. This method was highly sensitive and required only 0.2 mg of rat liver tissues for the measurement of the activity. It was reproducible and can be applied for the simultaneous assay of many samples. The activity of GTP cyclohydrolase I was measured in several rat tissues. For example, the enzyme activity in rat striatum (n = 5) was 13.7 +/- 1.5 pmol/mg protein per hour (mean +/- SE), and agreed well with those obtained by high-performance liquid chromatography with fluorescence detection. The activity in the autopsy human brains (caudate nucleus) was measured by this new method for the first time. The activity in the caudate nucleus from parkinsonian patients (n = 6) was 0.82 +/- 0.56 pmol/mg protein per hour which was significantly lower than the control value, 4.22 +/- 0.43 pmol/mg protein per hour (n = 10).

Research Organization:
Tokyo Institute of Technology, Japan
OSTI ID:
5528037
Journal Information:
Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Journal Issue: 1 Vol. 154:1; ISSN ANBCA
Country of Publication:
United States
Language:
English

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Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
AUTOPSY
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CHROMATOGRAPHY
DATA
DIAGNOSIS
DIAGNOSTIC TECHNIQUES
DIGESTIVE SYSTEM
DISEASES
ENZYME ACTIVITY
ENZYMES
EXPERIMENTAL DATA
FLUORESCENCE
GLANDS
HYDROLASES
IMMUNOASSAY
IMMUNOLOGY
INFORMATION
ISOTOPE APPLICATIONS
LIQUID COLUMN CHROMATOGRAPHY
LIVER
LUMINESCENCE
MAMMALS
NERVOUS SYSTEM
NERVOUS SYSTEM DISEASES
NUMERICAL DATA
ORGANS
PATIENTS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RATS
RODENTS
SENSITIVITY
SEPARATION PROCESSES
TRACER TECHNIQUES
VERTEBRATES