Radioimmunoassay for herpes simplex virus (HSV) thymidine kinase

Journal Article · Fri Jan 29 23:00:00 EST 1982 · Virology; (United States)

DOI:https://doi.org/10.1016/0042-6822(82)90142-8· OSTI ID:5527294

McGuirt, P V; Keller, P M; Elion, G B

A sensitive RIA for HSV-1 thymidine kinase (TK) has been developed. This assay is based on competition for the binding site of a rabbit antibody against purified HSV-1 TK, between a purified /sup 3/H-labeled HSV-1 TK and a sample containing an unknown amount of viral TK. The assay is capable of detecting 8 ng or more of the HSV enzyme. Purified HSV-1 TK denatured to <1% of its original kinase activity is as effective in binding to the antibody as is native HSV-1 TK. Viral TK is detectable at ranges of 150-460 ng/mg protein of cell extract from infected cells or cells transformed by HSV or HSV genetic material. HSV-2 TK appears highly cross-reactive, VZV TK is slightly less so, and the vaccinia TK shows little or no cross-reactivity. This RIA may serve as a tool for monitoring the expression of the HSV TK during an active herpes virus infection, a latent ganglionic infection, or in neoplastic cells which may have arisen by viral transformation.

• Research Organization: Burroughs Wellcome Co., Research Triangle Park, NC
• OSTI ID: 5527294
• Journal Information: Virology; (United States), Journal Name: Virology; (United States) Vol. 116:2; ISSN VIRLA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIBODIES
BIOCHEMICAL REACTION KINETICS
COMPETITION
DISEASES
ENZYME ACTIVITY
ENZYMES
HERPES SIMPLEX
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
KINETICS
MICROORGANISMS
PARASITES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
RADIOASSAY
RADIOIMMUNOASSAY
REACTION KINETICS
SKIN DISEASES
TRACER TECHNIQUES
TRANSFERASES
VIRAL DISEASES
VIRUSES