Glial-released proteins in clonal cultures and their modulation by hydrocortisone
Rat glial C6 cells release into the culture medium a reproducible spectrum of soluble proteins of 12 major peaks over a broad molecular weight range as determined by fractionation on SDS-gel electrophoresis. Exposing C6 monolayers to hydrocortisone (HC) results in a selective alteration in the pattern of glial-released protein (GRP). The selective HC-induced increase or decrease in GRP peaks is specific to HC in that 17 ..beta..-estradiol, dibutyryl cyclic AMP, isoproterenol, and melatonin exert either no detectable or a qualitatively different influence on the GRP pattern. The HC influence is dose dependent over a physiological range of concentrations from 10/sup -9/ to 10/sup -6/ M. Differences in culture age and in subclones of C6 can influence both the normal and the HC-induced pattern of GRP. The origin of the GRP is unknown, but pattern reproducibility, viability tests, surface labelling studies, and metabolic labelling studies of soluble and particulate compartment proteins and glycoproteins support the position that cell lysis is not an important source of GRP. More importantly, these studies indicate that GRP and HC-induced changes in GRP pattern are physiologically significant aspects of glial cell behavior.
- Research Organization:
- California Univ., Los Angeles (USA). Lab. of Nuclear Medicine and Radiation Biology
- DOE Contract Number:
- EY-76-C-03-0012
- OSTI ID:
- 5525768
- Report Number(s):
- UCLA-12-1233
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
HYDROCORTISONE
BIOLOGICAL EFFECTS
NERVE CELLS
PHYSIOLOGY
PROTEINS
SECRETION
CELL CULTURES
EXPERIMENTAL DATA
ISOLATED VALUES
RATS
ADRENAL HORMONES
ANIMAL CELLS
ANIMALS
CORTICOSTEROIDS
DATA
DATA FORMS
GLUCOCORTICOIDS
HORMONES
HYDROXY COMPOUNDS
INFORMATION
KETONES
MAMMALS
NUMERICAL DATA
ORGANIC COMPOUNDS
PREGNANES
RODENTS
SOMATIC CELLS
STEROID HORMONES
STEROIDS
VERTEBRATES
550300* - Cytology
551000 - Physiological Systems