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Title: Continuous human cell lines and method of making same

Abstract

Substantially genetically stable continuous human cell lines derived from normal human mammary epithelial cells (HMEC) and processes for making and using the same. In a preferred embodiment, the cell lines are derived by treating normal human mammary epithelial tissue with a chemical carcinogen such as benzo(a)pyrene. The novel cell lines serve as useful substrates for elucidating the potential effects of a number of toxins, carcinogens and mutagens as well as of the addition of exogenous genetic material. The autogenic parent cells from which the cell lines are derived serve as convenient control samples for testing. The cell lines are not neoplastically transformed, although they have acquired several properties which distinguish them from their normal progenitors. 2 tabs.

Inventors:
Publication Date:
Research Org.:
Lawrence Berkeley Lab., CA (USA)
OSTI Identifier:
5524665
Application Number:
ON: DE86013863
Assignee:
Dept. of Energy LBNL; ERA-11-005674; EDB-86-139173
DOE Contract Number:  
AC03-76SF00098
Resource Type:
Patent
Resource Relation:
Other Information: Portions of this document are illegible in microfiche products
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; CARCINOGEN SCREENING; DESIGN; CLONE CELLS; CELL PROLIFERATION; MUTAGEN SCREENING; BENZOPYRENE; BIOASSAY; CELL CULTURES; MAMMARY GLANDS; MAN; ANIMALS; AROMATICS; BODY; CONDENSED AROMATICS; GLANDS; HYDROCARBONS; MAMMALS; ORGANIC COMPOUNDS; ORGANS; PRIMATES; SCREENING; VERTEBRATES; 560300* - Chemicals Metabolism & Toxicology; 550300 - Cytology

Citation Formats

Stampfer, M.R.. Continuous human cell lines and method of making same. United States: N. p., 1985. Web.
Stampfer, M.R.. Continuous human cell lines and method of making same. United States.
Stampfer, M.R.. Mon . "Continuous human cell lines and method of making same". United States.
@article{osti_5524665,
title = {Continuous human cell lines and method of making same},
author = {Stampfer, M.R.},
abstractNote = {Substantially genetically stable continuous human cell lines derived from normal human mammary epithelial cells (HMEC) and processes for making and using the same. In a preferred embodiment, the cell lines are derived by treating normal human mammary epithelial tissue with a chemical carcinogen such as benzo(a)pyrene. The novel cell lines serve as useful substrates for elucidating the potential effects of a number of toxins, carcinogens and mutagens as well as of the addition of exogenous genetic material. The autogenic parent cells from which the cell lines are derived serve as convenient control samples for testing. The cell lines are not neoplastically transformed, although they have acquired several properties which distinguish them from their normal progenitors. 2 tabs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Jul 01 00:00:00 EDT 1985},
month = {Mon Jul 01 00:00:00 EDT 1985}
}