Continuous human cell lines and method of making same
Substantially genetically stable continuous human cell lines derived from normal human mammary epithelial cells (HMEC) and processes for making and using the same. In a preferred embodiment, the cell lines are derived by treating normal human mammary epithelial tissue with a chemical carcinogen such as benzo(a)pyrene. The novel cell lines serve as useful substrates for elucidating the potential effects of a number of toxins, carcinogens and mutagens as well as of the addition of exogenous genetic material. The autogenic parent cells from which the cell lines are derived serve as convenient control samples for testing. The cell lines are not neoplastically transformed, although they have acquired several properties which distinguish them from their normal progenitors. 2 tabs.
- Research Organization:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- DOE Contract Number:
- AC03-76SF00098
- Assignee:
- Dept. of Energy
- Application Number:
- ON: DE86013863
- OSTI ID:
- 5524665
- Resource Relation:
- Other Information: Portions of this document are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
CARCINOGEN SCREENING
DESIGN
CLONE CELLS
CELL PROLIFERATION
MUTAGEN SCREENING
BENZOPYRENE
BIOASSAY
CELL CULTURES
MAMMARY GLANDS
MAN
ANIMALS
AROMATICS
BODY
CONDENSED AROMATICS
GLANDS
HYDROCARBONS
MAMMALS
ORGANIC COMPOUNDS
ORGANS
PRIMATES
SCREENING
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550300 - Cytology