Indomethacin and salicylate decrease epinephrine-induced glycogenolysis

Journal Article · Fri Feb 01 04:00:00 EST 1985 · Metabolism; (United States)

DOI:https://doi.org/10.1016/0026-0495(85)90124-6· OSTI ID:5522952

Miller, J D; Ganguli, S; Artal, R; Sperling, M A

Epinephrine (E) produces an immediate (0-30 minutes) rise in hepatic glucose production (Ra), largely due to activation of glycogenolysis; thereafter, E-stimulated gluconeogenesis becomes the major factor maintaining glucose production. To investigate the possible role of arachidonic acid metabolites on Ra during E stimulation, the authors infused E in trained conscious dogs before and during administration of two inhibitors of arachidonic acid metabolism, indomethacin (INDO) and salicylate (S). On separate days, experimental animals were treated with both oral and IV INDO and oral acetylsalicylic acid and IV sodium salicylate. Ra and glucose utilization (Rd), both in mg x kg-1 min-1, were calculated by isotope dilution using 3-/sup 3/H-glucose. After achieving steady state specific activity, control (C) and experimental animals (n . 6 per group) received E (0.1 ug x kg-1 min-1) for 150 minutes, raising plasma levels to approximately 1500 pg/mL in each group. In C, plasma glucose (G; mg/dL) rose by 17 +/- 5 at 10 minutes and 19 +/- 3 at 20 minutes due to an initial spike in Ra (2.7 +/- 0.2 to 4.9 +/- 0.5; P less than 0.01) at 10 minutes. INDO and S treatment attenuated this initial (10-20 minutes) rise in G (P less than 0.05) due to a lower stimulated Ra at 10 minutes (3.3 +/- 0.1 with INDO; 3.0 +/- 0.5 with S; P less than 0.05). After 20 minutes Ra was not different in the 3 groups; no overall differences in Rd, glucose clearance, or plasma insulin levels occurred with INDO or S treatment.

• Research Organization: Univ. of Cincinnati, Ohio
• OSTI ID: 5522952
• Journal Information: Metabolism; (United States), Journal Name: Metabolism; (United States) Vol. 34:2; ISSN MTBMA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETIC ACID
ADRENAL HORMONES
ADRENALINE
ALDEHYDES
ANIMALS
ARACHIDONIC ACID
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BLOOD-PLASMA CLEARANCE
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CARDIOTONICS
CARDIOVASCULAR AGENTS
CLEARANCE
DIGESTIVE SYSTEM
DOGS
DRUGS
GLANDS
GLUCOSE
HEXOSES
HORMONES
HYDROXY ACIDS
INSULIN
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
LABELLED COMPOUNDS
LIVER
MAMMALS
METABOLISM
METABOLITES
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
NEUROREGULATORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
SACCHARIDES
SALICYLIC ACID
STEROID HORMONES
SYMPATHOMIMETICS
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES