Microvascular albumin permeability in isolated perfused lung: effects of EDTA

Journal Article · Fri Feb 01 04:00:00 EST 1985 · J. Appl. Physiol.; (United States)

OSTI ID:5520656

Kern, D F; Malik, A B

The authors examined the effects of decreases in perfusate concentrations of calcium and magnesium on the pulmonary vascular permeability in the isolated perfused rabbit lung. The albumin permeability-surface area product (PS) and the albumin reflection coefficient (sigma) were determined in the same lung using /sup 125/I- and /sup 131/I-labeled albumin tracers. Decreases in vascular Ca/sup 2 +/ and Mg/sup 2 +/ concentrations were induced by adding ethylenediaminetetraacetic acid (EDTA) to the perfusate. Decreases in the concentration of these cations resulted in an increase in the PS from a control value of 1.18 +/- 0.13 X 10(-3) to 7.69 +/- 0. 75 X 10(-3) cm3 X min-1 X g wet lung wt-1 and a decrease in the sigma from 0.96 +/- 0.01 to 0.74 +/- 0.02. The decrease in sigma suggests an increase in the calculated equivalent pore radius from 44 to 63 A. The results indicate that Ca/sup 2 +/ and Mg/sup 2 +/ play a role in the maintenance of normal pulmonary vascular permeability to proteins.

• Research Organization: Albany Medical College, New York, NY
• OSTI ID: 5520656
• Journal Information: J. Appl. Physiol.; (United States), Journal Name: J. Appl. Physiol.; (United States) Vol. 58:2; ISSN JAPYA
• Country of Publication: United States
• Language: English

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550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
ALKALINE EARTH METAL COMPOUNDS
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BLOOD VESSELS
BODY
CALCIUM COMPOUNDS
CAPILLARIES
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CATIONS
CHARGED PARTICLES
CHELATING AGENTS
DAYS LIVING RADIOISOTOPES
EDTA
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE 131
IODINE ISOTOPES
IONS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LUNGS
MAGNESIUM COMPOUNDS
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PERFUSED ORGANS
PERMEABILITY
PROTEINS
RABBITS
RADIOISOTOPES
RESPIRATORY SYSTEM
TRACER TECHNIQUES
VERTEBRATES