Mutagenic effect of radionuclides incorporated into DNA of Drosophila melanogaster. Progress report, July 15, 1984-July 15, 1985

Technical Report · Tue Jan 01 04:00:00 EST 1985

OSTI ID:5520554

Lee, W R

We have found that the most efficient analysis of mutants is carried out by using both the mutant protein and DNA. A sequential analysis of mutants at the molecular level has been developed. We found that of the seven apparently intragenic mutations disclosed, only three had normal southern blots and, as seen in two dimensional gels, only one of these produced a protein with a subunit which had normal molecular weight and electrical charge. The importance of this finding is that the molecular analysis was able to detect deletions missed by conventional genetic analysis even in a well developed genetic test system like Drosophila melanogaster. 4 refs., 1 fig. (DT)

• Research Organization: Louisiana State Univ., Baton Rouge (USA). Dept. of Zoology and Physiology
• DOE Contract Number: AS05-76EV03728
• OSTI ID: 5520554
• Report Number(s): DOE/EV/03728-18; ON: DE85016110
• Country of Publication: United States
• Language: English

Related Subjects

550401* -- Genetics-- Tracer Techniques
560304 -- Chemicals Metabolism & Toxicology-- Invertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ARTHROPODS
BIOLOGICAL EFFECTS
CELL CONSTITUENTS
DIPTERA
DNA
DROSOPHILA
ENZYMES
FLIES
FRUIT FLIES
GENES
GENETIC EFFECTS
INSECTS
INVERTEBRATES
MITOCHONDRIA
MUTANTS
NITROSO COMPOUNDS
NITROSOUREAS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
OXIDOREDUCTASES
RECOMBINANT DNA