Polyphophoinositides components of plant nuclear membranes

Conference · Wed Apr 01 04:00:00 EST 1987 · Plant Physiol., Suppl.; (United States)

OSTI ID:5520546

Hendrix, K W; Boss, W F

The polyphosphoinositides, phosphatidylinositol monophosphate (PIP) and phosphatidylinositol bisphosphate (PIP/sub 2/), have been shown to be important components in signal transduction in many animal cells. Recently, these lipids have been found to be associated with plasma membrane but not microsomal membrane isolated from fusogenic wild carrot cells; however, in that study the lipids of the nuclear membrane were not analyzed. Since polyphosphoinositides had been shown to be associated with the nuclear membranes as well as the plasma membrane in some animal cells, it was important to determine whether they were associated with plant nuclear membranes as well. Cells were labeled for 18h with (/sup 3/H) inositol and the nuclei were isolated by a modification of the procedure of Saxena et al. Preliminary lipid analyses indicate lower amount of PIP and PIP/sub 2/ in nuclear membranes compared to whole protoplasts. This suggests that the nuclear membranes of carrot cells are not enriched in PIP and PIP/sub 2/; however, the Triton X-100 used during the nuclear isolation procedure may have affected the recovery of the lipids. Experiments are in progress to determine the effects of Triton X-100 on lipid extraction.

• Research Organization: North Carolina State Univ., Raleigh
• OSTI ID: 5520546
• Report Number(s): CONF-8707108-
• Conference Information: Journal Name: Plant Physiol., Suppl.; (United States) Journal Volume: 83:4
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADDITIVES
CARBOHYDRATES
CARROTS
CELL CONSTITUENTS
CELL MEMBRANES
CELL NUCLEI
CHEMICAL COMPOSITION
DETERGENTS
EMULSIFIERS
ESTERS
EXTRACTION
FOOD
INOSITOL
INOSITOLS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
MEMBRANES
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
PLANTS
SACCHARIDES
SEPARATION PROCESSES
SURFACTANTS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VEGETABLES
WETTING AGENTS