Polyphosphoinositides are present in plasma membranes isolated from fusogenic carrot cells

Journal Article · Thu Oct 01 04:00:00 EDT 1987 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.85.2.389· OSTI ID:5640055

Wheeler, J J; Boss, W F

Fusogenic carrot cells grown in suspension culture were labeled 12 hours with myo-(2-/sup 3/H)inositol. Plasma membranes were isolated from the prelabeled fusogenic carrot cells by both aqueous polymer two-phase partitioning and Renografin density gradients. With both methods, the plasma membrane-enriched fractions, as identified by marker enzymes, were enriched in (/sup 3/H)inositol-labeled phosphatidylinositol monophosphate (PIP) and phosphatidylinositol bisphosphate (PIP/sub 2/). An additional (/sup 3/H)inositol-labeled lipid, lysophosphatidylinositol monophosphate, which migrated between PIP and PIP/sub 2/ on thin layer plates, was found primarily in the plasma membrane-rich fraction of the fusogenic cells. This was in contrast to lysophosphatidylinositol which is found primarily in the lower phase, microsomal/mitchrondrial-rich fraction.

• Research Organization: North Carolina State Univ., Raleigh
• OSTI ID: 5640055
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 85:2; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL LOCALIZATION
CARBOHYDRATES
CARROTS
CELL CONSTITUENTS
CELL CULTURES
CELL MEMBRANES
ESTERS
FOOD
INOSITOL
INOSITOLS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
MEMBRANES
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
PLANT CELLS
PLANTS
SACCHARIDES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VEGETABLES