Automated single-cell manipulation and sorting by light trapping

Journal Article · Tue Dec 15 00:00:00 EST 1987 · Appl. Opt.; (United States)

DOI:https://doi.org/10.1364/AO.26.005311· OSTI ID:5520386

Buican, T N; Smyth, M J; Crissman, H A; Salzmann, G C; Stewart, C C; Martin, J C

Following the recently reported trapping of biological particles by finely focused laser beams, we report on the automated micromanipulation of cells and other microscopic particles by purely optical means as well as on a newly observed interaction between particles in the trapping beam. A simple instrument is described which allows single cells to be positioned with high accuracy, transported over several millimeters, and automatically sorted on the basis of their optical properties. These operations are performed inside a small enclosed chamber without mechanical contact or significant fluid flow. Potential applications of this technique in experimental cell biology are discussed.

• Research Organization: Los Alamos National Laboratory, Los Alamos, New Mexico 87545
• OSTI ID: 5520386
• Journal Information: Appl. Opt.; (United States), Vol. 26:24
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
47 OTHER INSTRUMENTATION
CYTOLOGY
OPTICAL EQUIPMENT
DESIGN
ANIMAL CELLS
AUTOMATION
BIOTECHNOLOGY
CELL FLOW SYSTEMS
CYTOLOGICAL TECHNIQUES
TRAPPING
BIOLOGY
EQUIPMENT
550300* - Cytology
440300 - Miscellaneous Instruments- (-1989)
550200 - Biochemistry