Repair of DNA treated with. gamma. -irradiation and chemical carcinogens. Progress report, March 15, 1979-March 15, 1980

Technical Report · Tue Jan 01 04:00:00 EST 1980

DOI:https://doi.org/10.2172/5520036· OSTI ID:5520036

Goldthwait, D. A.

The identification and purification of 3-methyladenine glycosylase from the cell nuclei of rat liver was completed. The characterization of 7-methylguanine N-glycosylase activity in E. coli is currently under investigation. Alkylated DNA in chromatin structures as a substrate for 3-methyladenine N-glycosylase is discussed. An enzyme from E. coli and mammalian tissue active on 2,6-diamino-4-hydroxy-5-N-methyldormamido-pyrimide is isolated and characterized. Studies are proceeding on the correlation of x-ray sensitivity with removal of alkylated bases from DNA in x-ray sensitive and x-ray resistant lines of lymphoma cells. The reaction of ..beta..-propiolactone with derivatives of adenine and with DNA is discussed.

• Research Organization: Case Western Reserve Univ., Cleveland, OH (USA). Dept. of Biochemistry
• DOE Contract Number: EY-76-S-02-2725
• OSTI ID: 5520036
• Report Number(s): COO-2725-05
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADENINES
ALKYLATION
AMINES
ANIMALS
BACTERIA
BODY
CHEMICAL REACTIONS
CHROMATIN
DIGESTIVE SYSTEM
DISEASES
DNA
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ENZYMES
ESCHERICHIA COLI
GLANDS
HETEROCYCLIC COMPOUNDS
IONIZING RADIATIONS
LIVER
LYMPHOMAS
MAMMALS
MICROORGANISMS
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PURIFICATION
PURINES
RADIATIONS
RADIOSENSITIVITY
RATS
RODENTS
VERTEBRATES
X RADIATION