Repair of DNA treated with. gamma. -irradiation and chemical carcinogens. Comprehensive report of entire period of ERDA support from June 1, 1975--January 15, 1978
A partially purified enzyme fraction isolated from E. coli showed an N-glycosidase activity as well as a phosphodiesterase activity on DNA treated with methylnitrosourea, and with 7-bromomethylbenz(a)anthracene and a phosphodiesterase activity against ..gamma..-irradiated DNA. Both 0-6 methyl guanine and 3-methyladenine were released from DNA treated with MNU; the adenine and guanine derivatives from the DNA treated with 7-bromomethyl-12-methylbenz(a)anthracene were also liberated. Progress is also reported on studies on Endonucleases II and VI and Exonuclease III of E. coli; methods for assay and for synthesis of substrates; attempts at purification of repair enzymes from mammalian tissues; and ..beta..-propiolactone reactions with deoxynucleosides and with DNA.
- Research Organization:
- Case Western Reserve Univ., Cleveland, OH (USA). Dept. of Biochemistry
- DOE Contract Number:
- EY-76-S-02-2725
- OSTI ID:
- 6931071
- Report Number(s):
- COO-2725-03
- Country of Publication:
- United States
- Language:
- English
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Repair of DNA treated with. gamma. -irradiation and chemical carcinogens. Progress report, February 1, 1976--February 28, 1977. [Endonuclease reactions in E. coli]
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Related Subjects
560111 -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CARCINOGENS
CHEMICAL RADIATION EFFECTS
CHEMISTRY
DNA
ENZYMES
ESCHERICHIA COLI
GAMMA SOURCES
HYDROLASES
KINETICS
MICROORGANISMS
MOLECULAR BIOLOGY
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATION SOURCES
RADIOINDUCTION
REACTION KINETICS
RECOVERY
REPAIR
STRAND BREAKS