Post-translational processing and secretion of atrial natriuretic factor

Thesis/Dissertation · Fri Jan 01 04:00:00 EST 1988

OSTI ID:5517897

Shields, P P

The post-translational processing and regulated secretion of atrial natriuretic factor (ANF) were studied in primary cultures of rat cardiac myocytes. Cultures were established from neonatal rat atria or ventricles, and were maintained for 7-14 days in complete serum free medium. The cultures contained high and constant levels of ANF-(1-126), the known storage form of the hormone in vivo. The cultures also secreted ANF-(1-126), instead of the known circulating form of the hormone, ANF-(99-126). However, the inclusion of the glucocorticoids dexamethasone or hydrocortisone in the culture medium increased the levels of ir-ANF contained and secreted by the cultures, and caused both atrial and ventricular cultures to secrete principally ANF-(99-126) instead of ANF-(1-126). The secreted peptide was shown to be authentic ANF-(99-126) by chromatographic, amino acid composition and radiosequence analysis, thus confirming that the cultures were accurately processing ANF to the in vivo circulating form in the presence of glucocorticoids. Glucocorticoids also caused an increase in size and clustering of atrial myocytes as determined by immunocytochemical analysis, but the morphological effects could be dissociated from the stimulation of ANF-(99-126) secretion by manipulating the timing of glucocorticoid exposure. The location of ANF-(99-126) formation was investigated using biosynthetically labeled {sup 35}S-Cys-ANF-(1-126) in conjunction with actively processing cultures.

• Research Organization: Pennsylvania Univ., Philadelphia, PA (USA)
• OSTI ID: 5517897
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
AMINO ACID SEQUENCE
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CARDIOVASCULAR SYSTEM
CELL CULTURES
CHROMATOGRAPHY
CORTICOSTEROIDS
CULTURE MEDIA
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
GLUCOCORTICOIDS
HEART
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
KINETICS
LIGHT NUCLEI
MAMMALS
MOLECULAR STRUCTURE
NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PREGNANES
PROTEINS
RADIOISOTOPES
RATS
REACTION KINETICS
RODENTS
SECRETION
SEPARATION PROCESSES
STEROIDS
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES
VERTEBRATES