Effect of various eicosanoid products of arachidonic acid on the acyl CoA: Cholesterol acyl transferase activity in three different mammalian cell lines
Thesis/Dissertation
·
OSTI ID:5516960
Acylcoenzyme A:cholesterol acyltransferase (ACAT) catalyzes cholesterol ester synthesis intracellularly and has been implicated in the development of atherosclerosis. An in vitro assay has been adapted for determining ACAT activity from rat FU5AH hepatoma, Chinese hamster ovary (CHO) and rat thoracic aortic smooth muscle (RSM) cells. Formation of {sup 14}C-labelled cholesteryl oleate at 0 to 60 min {plus minus} cholesterol was determined; in the presence of exogenous cholesterol, ACAT activity was approximately linear and surpassed the plateau observed in ACAT activity without cholesterol. Increasing exogenous cholesterol concentration, the amount of oleoyl CoA or the amount of microsomal protein produced a corresponding increase in ACAT activity, while ester formation was slightly increased by decreasing the ratio of Triton WR-1339 to cholesterol. Both the thromboxane A{sub 2} (TxA{sub 2}) mimic, U-44069, and the inflammatory lipoxygenase product, LTB{sub 4}, decreased optimal in vitro microsomal ACAT activity from RSM, but not form FU5AH, while CHO ACAT activity was suppressed by LTB{sub r} only. PGI{sub 2}, PGE{sub 2} and PGF{sub 2{alpha}} had minimal effects for each cell type.
- Research Organization:
- Drexel Univ., Philadelphia, PA (USA)
- OSTI ID:
- 5516960
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ARACHIDONIC ACID
ARTERIOSCLEROSIS
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
CHO CELLS
CHOLESTEROL
DISEASES
EICOSANOIC ACID
ENZYME ACTIVITY
ENZYMES
ESTERS
HEPATOMAS
HYDROXY COMPOUNDS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MONOCARBOXYLIC ACIDS
MUSCLES
NEOPLASMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PATHOGENESIS
RADIOASSAY
RATS
REACTION KINETICS
RODENTS
STEROIDS
STEROLS
SYNTHESIS
TRANSFERASES
VASCULAR DISEASES
VERTEBRATES
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ARACHIDONIC ACID
ARTERIOSCLEROSIS
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
CHO CELLS
CHOLESTEROL
DISEASES
EICOSANOIC ACID
ENZYME ACTIVITY
ENZYMES
ESTERS
HEPATOMAS
HYDROXY COMPOUNDS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MONOCARBOXYLIC ACIDS
MUSCLES
NEOPLASMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PATHOGENESIS
RADIOASSAY
RATS
REACTION KINETICS
RODENTS
STEROIDS
STEROLS
SYNTHESIS
TRANSFERASES
VASCULAR DISEASES
VERTEBRATES