Oncogene v-src transforms and established embryonic rodent fibroblasts but not diploid human fibroblasts

Journal Article · Wed Jun 01 04:00:00 EDT 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.12.4355· OSTI ID:5516678

Hjelle, B; Liu, E; Bishop, J M ^[1]

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1. Univ. of California, San Francisco (USA)

The conversion of cells from a normal phenotype to full malignancy apparently requires multiple genetic events. Efforts to reconstruct multistep tumorigenesis in cell culture have shown that two types of oncogenes (typified by HRAS and MYC) can cooperate to elicit complete transformation. Transformation of embryonic rodent cells by single oncogenes is reputed either not to occur or to require specialized circumstances. It has not been known how the large group of oncogenes that encode protein-tyrosine kinases might fit into this scheme. The authors now report that v-src, a prototype for the kinase oncogenes, can convert rat embryo fibroblasts to a fully transformed and tumorigenic phenotype when the gene is expressed vigorously. By contrast, v-src had no demonstrable effect on diploid human fibroblasts. The results sustain the view that it is possible for at least some oncogenes to achieve a potency sufficient for unilateral tumorigenesis.

• OSTI ID: 5516678
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 85:12; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
CARBOXYLIC ACIDS
CARCINOGENESIS
CELL TRANSFORMATIONS
CONNECTIVE TISSUE CELLS
EMBRYONIC CELLS
ENZYMES
ETIOLOGY
FIBROBLASTS
GENES
HYDROXY ACIDS
MOLECULAR BIOLOGY
ONCOGENES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PATHOGENESIS
PHENOTYPE
SOMATIC CELLS
TRANSFERASES
TYROSINE