A deuterium and carbon nuclear magnetic resonance spectroscopic investigation of blood flow and carbohydrate metabolism
The purpose of this study is the development and application of nuclear magnetic resonance (NMR) spectroscopic techniques for this study of whole tissue metabolism, tissue perfusion and blood flow. The feasibility of spin imaging deuterium-enriched tissue water is demonstrated in cat brain in vivo and in situ. The potential application of D{sub 2}O administration to deuterium-flow-imaging is considered. NMR investigations of hepatic carbohydrate metabolism were performed in rat liver in vivo and in situ. A coaxial, double-surface-coil, double-resonance probe was developed for carbon detection while decoupling neighboring proton scalar interactions ({sup 13}C-({sup 1}H)) in hepatic tissue within the living animal. Hormonal and substrate regulation of hepatic glucose and glycogen metabolism was investigated by monitoring the metabolic fate of an administered c-dose of (1-{sup 13}C)glucose. Label flux was directed primarily into newly-synthesized {sup 13}C-labeled glycogen. A multiple resonance ({sup 1}H, {sup 13}C, {sup 31}P) liver perfusion probe was designed for complimentary carbohydrate metabolic studies in rat liver in vitro. A description of the {sup 13}C-({sup 1}H)/{sup 31}P NMR perfusion probe is given. The surgical technique used for liver excision and peripheral life-support apparatus required to maintain hepatic function are also detailed.
- Research Organization:
- Washington Univ., Seattle, WA (USA)
- OSTI ID:
- 5516513
- Country of Publication:
- United States
- Language:
- English
Similar Records
Use of deuterium labelled glucose in evaluating the pathway of hepatic glycogen synthesis
/sup 13/C and /sup 31/P NMR study of gluconeogenesis: utilization of /sup 13/C-labeled substrates by perfused liver from streptozotocin-diabetic and untreated rats
Related Subjects
550600 -- Medicine
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ALDEHYDES
ANIMALS
BARYONS
BLOOD FLOW
BODY
BRAIN
CARBOHYDRATES
CARBON 13
CARBON ISOTOPES
CATS
CENTRAL NERVOUS SYSTEM
DEUTERIUM
DIAGNOSTIC TECHNIQUES
DIGESTIVE SYSTEM
ELEMENTARY PARTICLES
EVEN-ODD NUCLEI
FERMIONS
GLANDS
GLUCOSE
HADRONS
HEXOSES
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIVER
MAGNETIC RESONANCE
MAMMALS
METABOLISM
MONOSACCHARIDES
NERVOUS SYSTEM
NMR IMAGING
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
NUCLEI
NUCLEONS
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PERFUSED ORGANS
PHOSPHORUS 31
PHOSPHORUS ISOTOPES
PROTONS
RESONANCE
SACCHARIDES
SPECTRA
STABLE ISOTOPES
TRACER TECHNIQUES
VERTEBRATES