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Use of deuterium labelled glucose in evaluating the pathway of hepatic glycogen synthesis

Journal Article · · Biochem. Biophys. Res. Commun.; (United States)
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Deuterium labelled glucose has been used to study the pathway of hepatic glycogen synthesis during the fasted-refed transition in rats. Deuterium enrichment of liver glycogen was determined using nuclear magnetic resonance as well as mass spectroscopy. Sixty minutes after oral administration of deuterated glucose to fasted rats, the portal vein blood was fully enriched with deuterated glucose. Despite this, less than half of the glucose molecules incorporated into liver glycogen contained deuterium. The loss of deuterium label from glucose is consistent with hepatic glycogen synthesis by an indirect pathway requiring prior metabolism of glucose. The use of deuterium labelled glucose may prove to be a useful probe to study hepatic glycogen metabolism. Its use may also find application in the study of liver glycogen metabolism in humans by a noninvasive means.

Research Organization:
Davis School of Medicine, Sacramento, CA (USA)
OSTI ID:
6154252
Journal Information:
Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 159:2; ISSN BBRCA
Country of Publication:
United States
Language:
English

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Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMALS
BIOLOGICAL MATERIALS
BIOLOGICAL PATHWAYS
BLOOD
BODY
BODY FLUIDS
CARBOHYDRATES
DEUTERIUM
DIGESTIVE SYSTEM
FASTING
GLANDS
GLUCOSE
GLYCOGEN
HEXOSES
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIVER
MAGNETIC RESONANCE
MAMMALS
MASS SPECTROSCOPY
MATERIALS
METABOLISM
MONOSACCHARIDES
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-ODD NUCLEI
ORAL ADMINISTRATION
ORGANIC COMPOUNDS
ORGANS
POLYSACCHARIDES
RATS
RESONANCE
RODENTS
SACCHARIDES
SPECTROSCOPY
STABLE ISOTOPES
TRACER TECHNIQUES
VERTEBRATES