cDNA cloning and sequencing of rat alpha sub 1 -macroglobulin

Journal Article · Tue Mar 03 04:00:00 EST 1992 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00123a020· OSTI ID:5515870

Waermegaard, B; Martin, N; Johansson, S ^[1]

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1. Biomedical Center, Uppsala (Sweden)

cDNA clones coding for the plasma protease inhibitor {alpha}{sub 1}-macroglobulin were isolated from a rat liver library. The obtained cDNA sequence contained 4701 nucleotides and had an open reading frame coding for a 1500 amino acid long protein, including a 24 amino acid signal peptide. The identity of the deduced protein sequence as {alpha}{sub 1}-macroglobulin was established by comparison with published peptide sequences of the protein. The mature protein shares 53% and 57% overall amino acid identity with the two other identified members of the rat {alpha}-macroglobulin family, {alpha}{sub 1}-inhibitor 3 and {alpha}{sub 2}-macroglobulin. A sequence typical for an internal thiol ester was identified. Of the 24 cysteines, 23 are conserved with {alpha}{sub 2}-macroglobulin. However, instead of the two most C-terminal cysteines in {alpha}{sub 2}-macroglobulin, which forms a disulfide bridge in the receptor binding domain, {alpha}{sub 1}-macroglobulin contains phenylalanine. One MRNA species hybridizing with the {alpha}{sub 1}-macroglobulin probe was observed in rat and mouse liver RNA ({approximately} 6.2 kb), whereas no corresponding transcript was detected in RNA from human liver.

• OSTI ID: 5515870
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 31:8; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD COAGULATION FACTORS
CARBOXYLIC ACIDS
CLONING
COAGULANTS
CYSTEINE
DAYS LIVING RADIOISOTOPES
DNA
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
DRUGS
ENZYME INHIBITORS
GLOBULINS
GLOBULINS-ALPHA
HEMATOLOGIC AGENTS
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
MAMMALS
MEMBRANE PROTEINS
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RATS
RECEPTORS
RECOMBINANT DNA
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
THIOLS
VERTEBRATES