Molecular analysis of human complement component C5: localization of the structural gene to chromosome 9

Journal Article · Tue Mar 08 04:00:00 EST 1988 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00405a012· OSTI ID:5409450

Wetsel, R A; Lemons, R S; Le Beau, M M; Barnum, S R; Noack, D; Tack, B F

A human C5 clone (pC5HG2) was isolated from a cDNA library constructed from Hep G2 mRNA. he DNA sequence showed that the pC5HG2 insert was comprised of 3309 base pairs of pro-C5 coding sequence and 404 base pairs of 3'-untranslated sequence. The derived amino acid sequence contained the entire coding sequence of the C5 ..cap alpha..-chain, the ..beta..-..cap alpha..-chain junction region, and 100 amino acids (approximately 50%) of the ..beta..-chain. Protein sequences of four C5 tryptic peptides were aligned exactly to this sequence and demonstrated that C5 synthesized and secreted by Hep G2 cells is probably identical with plasma-derived C5. Coding sequence alignment of the human C5 sequences with those of murine C5 indicated that 80% of the nucleotides and 79% of the amino acids were placed identically in the two species. Amino acid sequence alignment of the homologous family members C3, C4, and ..cap alpha../sub 2/-macroglobulin with that of C5 demonstrated 27%, 25%, and 19% identity, respectively. As was found in murine C5, the corresponding thiol ester region of human C5 contained several conserved amino acids, but the critical cysteine and glutamine residues which give rise to the intramolecular thiol ester bond in C3, C4, and ..cap alpha../sub 2/-macroglobulin were absent in C5, having been replaced by serine and alanine, respectively. With the use of a panel of hamster-human somatic cell hybrids, the C5 gene was mapped to human chromosome 9. In situ chromosomal hybridization studies employing metaphase cells further localized the gene to bands 9q32-34, with the largest cluster of grains at 9q34.1.

• Research Organization: Research Institute of Scripps Clinic, La Jolla, CA (USA)
• OSTI ID: 5409450
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 27:5; ISSN BICHA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHROMOSOMES
COMPLEMENT
DAYS LIVING RADIOISOTOPES
DNA
DNA SEQUENCING
EVEN-ODD NUCLEI
GENES
GENETIC MAPPING
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAN
MAPPING
MESSENGER-RNA
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
RECOMBINANT DNA
RNA
STRUCTURAL CHEMICAL ANALYSIS
SULFUR 35
SULFUR ISOTOPES
VERTEBRATES