Identification of a umuDC locus in Salmonella typhimurium LT2

Journal Article · Sat Jul 01 04:00:00 EDT 1989 · Journal of Bacteriology; (USA)

OSTI ID:5513717

Smith, C M; Eisenstadt, E ^[1]

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1. Harvard Univ. School of Public Health, Boston, MA (USA)

The umuDC operon of Escherichia coli is required for efficient mutagenesis by UV light and many other DNA-damaging agents. The existence of a umuDC analog in Salmonella typhimurium has been questioned. With DNA probes to the E. coli umuD and umuC genes, we detected, by Southern blot hybridization, sequences similar to both of these genes in S. typhimurium LT2. We also confirmed that the presence of cloned E. coli umuD enhances the UV mutability and resistance of S. typhimurium. Our data strongly suggest that S. typhimurium contains a functional umuDC operon.

• OSTI ID: 5513717
• Journal Information: Journal of Bacteriology; (USA), Journal Name: Journal of Bacteriology; (USA) Vol. 171:7; ISSN JOBAA; ISSN 0021-9193
• Country of Publication: United States
• Language: English

Related Subjects

560130* -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHROMOSOMES
DNA HYBRIDIZATION
DNA REPAIR
DNA SEQUENCING
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
GENE MUTATIONS
GENE OPERONS
GENETIC MAPPING
HYBRIDIZATION
MAPPING
MICROORGANISMS
MOLECULAR BIOLOGY
MUTAGENESIS
MUTATIONS
RADIATIONS
RECOVERY
REPAIR
SALMONELLA
SALMONELLA TYPHIMURIUM
STRUCTURAL CHEMICAL ANALYSIS
ULTRAVIOLET RADIATION