Cloning of Salmonella typhimurium DNA encoding mutagenic DNA repair

Journal Article · Wed Nov 01 04:00:00 EST 1989 · Journal of Bacteriology; (USA)

OSTI ID:5099548

Thomas, S M; Sedgwick, S G ^[1]

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1. National Institute for Medical Research, Mill Hill, London (England)

Mutagenic DNA repair in Escherichia coli is encoded by the umuDC operon. Salmonella typhimurium DNA which has homology with E. coli umuC and is able to complement E. coli umuC122::Tn5 and umuC36 mutations has been cloned. Complementation of umuD44 mutants and hybridization with E. coli umuD also occurred, but these activities were much weaker than with umuC. Restriction enzyme mapping indicated that the composition of the cloned fragment is different from the E. coli umuDC operon. Therefore, a umu-like function of S. typhimurium has been found; the phenotype of this function is weaker than that of its E. coli counterpart, which is consistent with the weak mutagenic response of S. typhimurium to UV compared with the response in E. coli.

• OSTI ID: 5099548
• Journal Information: Journal of Bacteriology; (USA), Journal Name: Journal of Bacteriology; (USA) Vol. 171:11; ISSN 0021-9193; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

560130* -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CONSTITUENTS
CLONING
DNA HYBRIDIZATION
DNA REPAIR
DNA-CLONING
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
GENE OPERONS
GENETIC MAPPING
GENOTYPE
HYBRIDIZATION
MAPPING
MICROORGANISMS
MUTATIONS
PLASMIDS
RADIATIONS
RECOVERY
REPAIR
SALMONELLA
SALMONELLA TYPHIMURIUM
ULTRAVIOLET RADIATION