Solution conformation of n-linked oligosaccharides

Journal Article · Tue Oct 20 04:00:00 EDT 1987 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00395a001· OSTI ID:5513166

Homans, S W; Dwek, R A; Rademacher, T W

The purpose of this review is to show how the current understanding of oligosaccharide solution conformations and dynamics has been furthered by recent developments in NMR methodology together with the use of energy calculations. First, a general strategy for the assignment of /sup 1/H NMR spectra of oligosaccharides is described. The use of these assignments for the interpretation of nuclear Overhauser effect (NOE) experiments is then discussed. The latter generate distance constraints, which, in conjunction with conformational energy calculations and molecular dynamics simulations, allow the structural and dynamic properties of a given oligosaccharide to be determined. Finally, the authors explore the extent to which the proposed recognition function of oligosaccharides can be rationalized in terms of these properties.

• Research Organization: Univ. of Oxford, England
• OSTI ID: 5513166
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 26:21; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
BARYONS
CARBOHYDRATES
CONFORMATIONAL CHANGES
ELEMENTARY PARTICLES
FERMIONS
HADRONS
MAGNETIC RESONANCE
MOLECULAR STRUCTURE
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
NUCLEONS
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
OVERHAUSER EFFECT
PROTONS
RESONANCE
SACCHARIDES
SPECTRA