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U.S. Department of Energy
Office of Scientific and Technical Information

Effects of environmental and experimental design factors on culturing and testing of Ceriodaphnia dubia

Technical Report ·
OSTI ID:5511443
EPA has developed a 7-day toxicity test to evaluate effects of effluents on Ceriodaphnia dubia survival and reproduction. This study evaluated effects of laboratory conditions and culturing and tests procedures on Ceriodaphnia survival and reproduction. Temperature, food concentration, chamber size, solution renewal frequency, light quality, illumination, photoperiod, water type, and test organism age were evaluated to determine how these various factors affected individual culturing success and acceptibility and reproducibility of toxicity test results. Test conditions proposed by EPA were evaluated by varying individual environmental or experimental design conditions to determine a range of responses for survival and reproduction under controlled conditions, both with and without a reference toxicant. For those conditions not specified by EPA (e.g., water hardness, light quality), a commonly used range of conditions was evaluated. 19 refs., 4 tabs.
Research Organization:
Electric Power Research Inst., Palo Alto, CA (USA); Battelle Columbus Div., OH (USA)
Sponsoring Organization:
EPRI
OSTI ID:
5511443
Report Number(s):
EPRI-EN-6468
Country of Publication:
United States
Language:
English