Suitability of different silver enhancement methods applied to 1 nm colloidal gold particles: an immunoelectron microscopic study
- Max-Planck-Institut fuer Biologie, Tuebingen (Federal Republic of Germany)
In order to exploit the recently introduced 1 nm gold colloids in routine electron microscopic labeling experiments, an efficient enhancement step for a better visualization of this small marker is a prerequisite. Efficiency and reproducibility of enhancement as well as growth homogeneity of gold particles were evaluated for three different silver intensifying solutions: silver lactate/hydroquinone/gum arabic, and the commercially available IntenSE M silver enhancement kit. The best results were obtained by using the silver lactate/hydroquinone/gum arabic mixture. The quality of enhancement of the IntenSE M kit was considerably increased by the addition of the protective colloid gum arabic.
- OSTI ID:
- 5509856
- Journal Information:
- Journal of Electron Microscopy Technique; (United States), Journal Name: Journal of Electron Microscopy Technique; (United States) Vol. 17:3; ISSN 0741-0581; ISSN JEMTE
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
BACTERIA
CARBOXYLIC ACID SALTS
COLLOIDS
DISPERSIONS
EFFICIENCY
ELECTRON MICROSCOPY
ELEMENTS
ESCHERICHIA COLI
GOLD ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LACTATES
METALS
MICROORGANISMS
MICROSCOPY
SILVER
TRACER TECHNIQUES
TRANSITION ELEMENTS