Macro creatine kinase: determination and differentiation of two types by their activation energies

Journal Article · Fri Jan 01 00:00:00 EST 1982 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5508293

Stein, W; Bohner, J; Steinhart, R; Eggstein, M

Determination of the MB isoenzyme of creatine kinase in patients with acute myocardial infarction may be disturbed by the presence of macro creatine kinase. The relative molecular mass of this form of creatine kinase in human serum is at least threefold that of the ordinary enzyme, and it is more thermostable. Here we describe our method for determination of macro creatine kinases and an easy-to-perform test for differentiating two forms of macro creatine kinase, based on their distinct activation energies. The activation energies of serum enzymes are mostly in the range of 40-65 kJ/mol of substrate. Unlike normal cytoplasmatic creatine kinases and IgG-linked CK-BB (macro creatine kinase type 1) a second form of macro creatine kinase (macro creatine kinase type 2) shows activation energies greater than 80 kJ/mol of substrate. The exact composition of macro creatine kinase type 2 is still unknown, but there is good reason to believe that it is of mitochondrial origin.

• Research Organization: Medizinische Universitaetsklinik Tuebingen, F.R.G.
• OSTI ID: 5508293
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Vol. 28:1
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
BLOOD SERUM
CHEMICAL ANALYSIS
PHOSPHOTRANSFERASES
ACTIVATION ENERGY
CLASSIFICATION
CREATINE
MYOCARDIAL INFARCTION
PATIENTS
AMINO ACIDS
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
DISEASES
ENERGY
ENZYMES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
TRANSFERASES
400102* - Chemical & Spectral Procedures