Preadipocyte proliferation is affected by norepinephrine
Beta-adrenergic stimulators are known to alter body composition profiles in experimental animals. The most prominent in vivo adaptations to beta-agonists are a decreased adipose tissue and concurrent increased muscle mass. Our lab has previously reported that norepinephrine - a beta/sub 1/-agonist - compromised preadipocyte growth in a primary cell culture system. Accordingly, this study continued to examine the effect of norepinephrine (NE) on in vitro preadipocyte proliferation. Stromalvascular cells, obtained from the inguinal pad of 4-5 week old Sprague-Dawley rats, were grown in culture for two weeks. Cells were treated with NE (1 ..mu..M) during the proliferative growth phase and labelled with /sup 3/H-thymidine on day 2 of culture. Following exponential growth, all cells were placed on a differentiation promoting media. Adipocytes (including differentiated preadipocytes) and stromal cells were separated using a density gradient, and /sup 3/H-thymidine was determined for both cell types. NE exposure for 2 or 4 days significantly reduced preadipocyte /sup 3/H-thymidine uptake, indicating an inhibition of mitotic growth. Future experiments will examine possible mechanism(s) involved in the catecholamine induced suppression of preadipocyte proliferation.
- Research Organization:
- Univ. of Georgia, Athens
- OSTI ID:
- 5508115
- Report Number(s):
- CONF-8604222-; TRN: 86-026625
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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FAT CELLS
CELL PROLIFERATION
NORADRENALINE
BIOLOGICAL EFFECTS
ANIMAL CELLS
CELL CULTURES
INHIBITION
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ADRENAL HORMONES
AUTONOMIC NERVOUS SYSTEM AGENTS
AZINES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CONNECTIVE TISSUE CELLS
DRUGS
HETEROCYCLIC COMPOUNDS
HORMONES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
NEUROREGULATORS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RIBOSIDES
SOMATIC CELLS
STEROID HORMONES
SYMPATHOMIMETICS
550201* - Biochemistry- Tracer Techniques