Preadipocyte proliferation is affected by norepinephrine

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5508115

Jones, D D; Hausman, G J; Martin, R J

Beta-adrenergic stimulators are known to alter body composition profiles in experimental animals. The most prominent in vivo adaptations to beta-agonists are a decreased adipose tissue and concurrent increased muscle mass. Our lab has previously reported that norepinephrine - a beta/sub 1/-agonist - compromised preadipocyte growth in a primary cell culture system. Accordingly, this study continued to examine the effect of norepinephrine (NE) on in vitro preadipocyte proliferation. Stromalvascular cells, obtained from the inguinal pad of 4-5 week old Sprague-Dawley rats, were grown in culture for two weeks. Cells were treated with NE (1 ..mu..M) during the proliferative growth phase and labelled with /sup 3/H-thymidine on day 2 of culture. Following exponential growth, all cells were placed on a differentiation promoting media. Adipocytes (including differentiated preadipocytes) and stromal cells were separated using a density gradient, and /sup 3/H-thymidine was determined for both cell types. NE exposure for 2 or 4 days significantly reduced preadipocyte /sup 3/H-thymidine uptake, indicating an inhibition of mitotic growth. Future experiments will examine possible mechanism(s) involved in the catecholamine induced suppression of preadipocyte proliferation.

• Research Organization: Univ. of Georgia, Athens
• OSTI ID: 5508115
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ANIMAL CELLS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZINES
BIOLOGICAL EFFECTS
CARDIOTONICS
CARDIOVASCULAR AGENTS
CELL CULTURES
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
DRUGS
FAT CELLS
HETEROCYCLIC COMPOUNDS
HORMONES
INHIBITION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
NEUROREGULATORS
NORADRENALINE
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RIBOSIDES
SOMATIC CELLS
STEROID HORMONES
SYMPATHOMIMETICS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS