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Microinjection of partially purified protein factor restores DNA damage specifically in group A of xeroderma pigmentosum cells

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
; ; ; ;
Microinjection of cell extracts prepared from both human placenta and HeLa cells into xeroderma pigmentosum (XP) cells of complementation group A restores unscheduled DNA synthesis (UDS) in these cells after UV irradiation. These cells also showed normal resistance to UV irradiation. The half-life of the factors in the cell extracts corresponding to the UDS activity (factor A) was 14 hr in XP cells of group A, and the maximal level of UDS was exerted 2 hr after microinjection. The factors were sensitive to protease treatment but not to RNase treatment and were found to be approx. = 160 and approx. = 90 kDa by gel filtration. These two fractions of the factor(s) acted specifically in XP cells of complementation group A among complementation groups A, B, C, D, F, G, and probably E and H.
Research Organization:
Osaka Univ., Japan
OSTI ID:
5507854
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 83:5; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550601 -- Medicine-- Unsealed Radionuclides in Diagnostics
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DNA
DNA REPAIR
ELECTROMAGNETIC RADIATION
FETAL MEMBRANES
HELA CELLS
HETEROCYCLIC COMPOUNDS
LABELLED COMPOUNDS
MAMMALS
MAN
MEMBRANES
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLACENTA
PRIMATES
PROTEINS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RIBOSIDES
THYMIDINE
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
VERTEBRATES
XP CELLS