Sensitivity of cultured lymphocytes from patients with nevoid basal cell carcinoma syndrome to ultraviolet light and phytohemagglutinin stimulation

Journal Article · Mon Jan 01 00:00:00 EST 1990 · Teratogenesis, Carcinogenesis, and Mutagenesis; (United States)

DOI:https://doi.org/10.1002/tcm.1770100603· OSTI ID:5506530

Ferraro, P; Celotti, L; Furlan, D; Pattarello, I; Peserico, A ^[1]

---

1. Department of Biology, Padova (Italy)

DNA repair and replication after in vitro UV irradiation were determined in cultured peripheral blood lymphocytes from 6 patients with nevoid basal cell carcinoma syndrome (NBCCS) and from a group of control donors. DNA repair synthesis (UDS) was measured in unstimulated lymphocytes by incubation with 3H-TdR in the presence of hydroxyurea for 3 and 6 h after UV irradiation (6-48 J/m2). DNA replication was measured in PHA-stimulated lymphocytes, UV-irradiated or mock-irradiated, by incubation with 3H-TdR for 24 h. The effect of the mitogen was followed during 5 days after stimulation by determining the incorporation of 3H-TdR, the increase of cell number, and the mitotic index. NBCCS and control lymphocytes showed equal sensitivity to UV light in terms of UDS and reduced response to PHA. On the contrary, the mitotic index and the number of cells in stimulated cultures were significantly lower in the affected subjects. These data suggest an altered progression along the cell cycle, which could be characteristic of stimulated NBCCS lymphocytes.

• OSTI ID: 5506530
• Journal Information: Teratogenesis, Carcinogenesis, and Mutagenesis; (United States), Vol. 10:6; ISSN 0270-3211
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
LYMPHOCYTES
RADIOSENSITIVITY
PHYTOHEMAGGLUTININ
BIOCHEMICAL REACTION KINETICS
CARCINOMAS
CELL CYCLE
DNA REPAIR
DNA REPLICATION
MITOTIC INDEX
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
AGGLUTININS
ANIMAL CELLS
ANTIBODIES
AZINES
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
DISEASES
ELECTROMAGNETIC RADIATION
HEMAGGLUTININS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LEUKOCYTES
MATERIALS
MITOGENS
MUCOPROTEINS
NEOPLASMS
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
POLYSACCHARIDES
PROTEINS
PYRIMIDINES
RADIATIONS
REACTION KINETICS
RECOVERY
REPAIR
RIBOSIDES
SACCHARIDES
SOMATIC CELLS
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture
550201 - Biochemistry- Tracer Techniques