Ozone-induced increases in substance P and 8-epi-prostaglandin F2 alpha in the airways of human subjects
- Baylor College of Medicine, Houston, TX (United States)
We are interested in the mechanisms of ozone-induced lung effects after short-term exposure and the relationship with subsequent pulmonary inflammation and disease. Our hypothesis is that ozone, as a powerful oxidant, will diminish the activity of neutral endopeptidase (NEP) in the airways of humans with resulting increased concentrations of neuropeptides such as substance P (SP). We have exposed seven (two women, five men) healthy, nonsmoking individuals (22 to 30 yr of age) to filtered air and ozone (0.25 ppm) for 1 h in an environmental chamber during heavy exercise. Bronchoscopy with airway lavage (AL) and bronchoalveolar lavage (BAL) was performed immediately after ozone exposure. The lavage samples were analyzed by enzyme immunoassay for SP and 8-epi-prostaglandin F2 alpha (8-epi-PGF2 alpha) (a marker for oxidative free radical reaction) and by radioimmunoassay for complement fragments. FEV1 had declined 12.4 +/- 1.9% (mean +/- SEM) as a result of ozone exposure. The AL concentration for SP and 8-epi-PGF2 alpha and BAL concentration of C3a after ozone exposure were significantly higher than after the filtered air exposure (P < 0.05). There was a significant correlation between SP and 8-epi-PGF2 alpha concentrations in the AL fluid (r2 = 0.89 and P < 0.05). There were no changes in C5a in either compartment or any of the mediators in the plasma samples. These results extend previous results from animal studies suggesting that ozone's mechanism of action is through an oxidative reaction resulting in a decreased activity of NEP in the airways with a subsequent increase in the concentration and activity of SP.
- OSTI ID:
- 5506201
- Journal Information:
- American Journal of Respiratory Cell and Molecular Biology; (United States), Journal Name: American Journal of Respiratory Cell and Molecular Biology; (United States) Vol. 9:5; ISSN 1044-1549; ISSN AJRBEL
- Country of Publication:
- United States
- Language:
- English
Similar Records
Effects of nitric acid gas alone or in combination with ozone on healthy volunteers
Effects of ozone on lung mechanics and cyclooxygenase metabolites in dogs
Related Subjects
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOASSAY
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
BODY
DIAGNOSTIC TECHNIQUES
ENZYME ACTIVITY
ENZYME IMMUNOASSAY
ENZYMES
HYDROLASES
IMMUNOASSAY
IMMUNOLOGY
INFLAMMATION
ISOTOPE APPLICATIONS
KINETICS
LUNGS
ORGANIC COMPOUNDS
ORGANS
OZONE
PATHOLOGICAL CHANGES
PEPTIDE HYDROLASES
PEPTIDES
PROSTAGLANDINS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
REACTION KINETICS
RESPIRATORY SYSTEM
SYMPTOMS
TRACER TECHNIQUES