Inhibition of myosatellite cell proliferation by gamma irradiation does not prevent the age-related increase of the number of dystrophin-positive fibers in soleus muscles of mdx female heterozygote mice

Journal Article · Sat Jun 01 00:00:00 EDT 1991 · American Journal of Pathology; (United States)

OSTI ID:5505783

Weller, B; Karpati, G; Lehnert, S; Carpenter, S; Ajdukovic, B; Holland, P ^[1]

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1. Neuromuscular Research Group, Montreal Neurological Institute, Quebec (Canada)

In skeletal muscles of young mdx female heterozygote mice, there is a mosaic of dystrophin-positive and dystrophin-negative fiber segments. In older animals, there is a marked decline in the number of dystrophin-negative fiber segments. This phenomenon might be due to a fusion of dystrophin-competent satellite cells into the originally dystrophin-negative fiber segments during growth. To study this possibility, soleus muscles of 10-day-old mdx female heterozygotes were gamma irradiated (2000 rads) to inhibit subsequent myosatellite cell proliferation and fusion. In the irradiated soleus muscles of animals at 60 days, the relative amount of dystrophin measured by quantitative immunoblots was not significantly different from that of the contralateral nonirradiated muscles. The prevalence of dystrophin-negative fibers in the 60-day-old irradiated solei was not higher than in the nonirradiated contralateral muscles, implying that dystrophin-competent satellite cell fusion was not a significant factor in the observed conversion. A longitudinal expansion of the cytoplasmic domain of the original dystrophin-competent myonuclei during growth could explain the observed conversion phenomenon.

• OSTI ID: 5505783
• Journal Information: American Journal of Pathology; (United States), Journal Name: American Journal of Pathology; (United States) Vol. 138:6; ISSN AJPAA; ISSN 0002-9440
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGE DEPENDENCE
ANIMAL CELLS
ANIMAL GROWTH
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL PROLIFERATION
DOSES
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
GROWTH
INHIBITION
IONIZING RADIATIONS
MAMMALS
MICE
MUSCLES
RADIATION DOSES
RADIATION EFFECTS
RADIATIONS
RODENTS
VERTEBRATES