Androgen uptake in the female reproductive system of a nonhuman primate
The nuclear uptake and retention of /sup 3/H-dehydrotestosterone (/sup 3/H-DHT) were studied in the reproductive organs of the female rhesus monkey by autoradiography. Ovariectomized and adrenalectomized adult female monkeys were injected with 1 microgram of /sup 3/H-DHT (107 Ci/mmole) and were exsanguinated 1 hour later. The uterus, cervix, vagina, and fimbriae of the fallopian tubes were removed and processed for autoradiography. Localization of the androgen was observed in the nuclei of fibroblasts, but not in the nuclei of smooth muscle and tissues of the blood vessels, of all four organs. The uptake of androgen by the epithelium varied for each organ. The simple columnar epithelium of the fallopian tube demonstrated nuclear labeling, but the epithelium of the uterus did not. The cells of the germinative layer of the stratified squamous epithelium of both the cervix and vagina exhibited nuclear concentration of label; however, the simple columnar epithelium of the cervix contiguous with the uterus was free of label. The results suggest that androgens may have a physiologic role in the female reproductive organs, and that the therapeutic effect of drugs such as danazol may be mediated via an androgen receptor.
- OSTI ID:
- 5503271
- Journal Information:
- Am. J. Obstet. Gynecol.; (United States), Journal Name: Am. J. Obstet. Gynecol.; (United States) Vol. 140:6; ISSN AJOGA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
551001 -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANDROGENS
ANDROSTANES
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BODY
CHEMICAL ANALYSIS
CONNECTIVE TISSUE CELLS
FEMALE GENITALS
FEMALES
FIBROBLASTS
HORMONES
LABELLED COMPOUNDS
MACACUS
MAMMALS
MONKEYS
ORGANIC COMPOUNDS
ORGANS
PHYSIOLOGY
PRIMATES
QUANTITATIVE CHEMICAL ANALYSIS
RETENTION
SOMATIC CELLS
STEROID HORMONES
STEROIDS
TRITIUM COMPOUNDS
UPTAKE
VERTEBRATES