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Differential effect of adriamycin on DNA replicative and repair synthesis in cultured neonatal rat cardiac cells

Journal Article · · Cancer Res.; (United States)
OSTI ID:5502956
; ;
The effect of the potent antitumor antiobiotic Adriamycin (ADM) on DNA replication and unscheduled DNA synthesis in cultured rat cardiac cells was investigated. Autoradiography and (3H)thymidine incorporation studies were carried out on parallel cultures. DNA replication was depressed for up to 6 days following a 3-h pulse of ADM administration. An ADM concentration of 1 microgram/ml which was effective in reducing replicative DNA synthesis by as much as 75% did not reduce the ability of cardiac cells to repair uv-damaged DNA. However, cells exposed to higher ADM concentrations failed to undergo significant uv-induced repair. In the absence of uv treatment, ADM did not stimulate unscheduled DNA synthesis. To account for the differential response of the cardiac cell cultures to replicate and repair DNA, we propose that ADM exerts a localized effect on DNA synthesis covering a region proximal to its primary intercalation site.
Research Organization:
Univ. of Southern California, Los Angeles
OSTI ID:
5502956
Journal Information:
Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 39:4; ISSN CNREA
Country of Publication:
United States
Language:
English

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Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTINEOPLASTIC DRUGS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
DNA
DNA REPLICATION
DOXORUBICIN
DRUGS
ELECTROMAGNETIC RADIATION
INHIBITION
IRRADIATION
MAMMALS
NEONATES
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY EFFECTS
RATS
RECOVERY
REPAIR
RODENTS
SOMATIC CELLS
ULTRAVIOLET RADIATION
VERTEBRATES