Correlation between ultraviolet survival and DNA repair efficiency in mouse cell hybrids and their parent lines
Journal Article
·
· Radiat. Res.; (United States)
Three hybrid cell lines formed between mouse lymphoma (LS) and mouse fibroblasts (A9) have been tested for their capacity to perform unscheduled DNA synthesis; their recovery characteristics after uv irradiation have also been studied to determine if DNA repair is implicated in the high survival observed in one hybrid (clone 3). The results of these investigations indicate that hybrid clone 3 was distinguishable from the more uv sensitive parental and other hybrid cell lines by its higher uv-induced unscheduled DNA synthesis, its greater clonogenic survival in plateau phase, and its faster recovery when maintained in conditioned medium after irradiation. The simultaneous increase of these three properties in hybrid clone 3 suggest that, by three different approaches, we have evidenced the same molecular process, a process involved in the elimination of potentially lethal damage, most probably the excision repair pathway. This report also shows that the low efficiency in excision repair in the parent line A9 is probably not due to deletion but rather to repression of the relevant gene(s) and that somatic cell hybridization can result in a stimulation of a previously poorly expressed repair process.
- Research Organization:
- Universite Libre de Bruxelles, Belgium
- OSTI ID:
- 5500869
- Journal Information:
- Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 90:1; ISSN RAREA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
CLONE CELLS
CONNECTIVE TISSUE CELLS
DISEASES
DNA
ELECTROMAGNETIC RADIATION
EXTERNAL IRRADIATION
FIBROBLASTS
HYBRIDIZATION
IRRADIATION
LYMPHOMAS
MAMMALS
MICE
MUTATIONS
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATIONS
RECOVERY
REPAIR
RODENTS
SOMATIC CELLS
SOMATIC MUTATIONS
STIMULATION
ULTRAVIOLET RADIATION
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
CLONE CELLS
CONNECTIVE TISSUE CELLS
DISEASES
DNA
ELECTROMAGNETIC RADIATION
EXTERNAL IRRADIATION
FIBROBLASTS
HYBRIDIZATION
IRRADIATION
LYMPHOMAS
MAMMALS
MICE
MUTATIONS
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATIONS
RECOVERY
REPAIR
RODENTS
SOMATIC CELLS
SOMATIC MUTATIONS
STIMULATION
ULTRAVIOLET RADIATION
VERTEBRATES