Phorbol esters alter the expression of lymphocyte membrane proteins
T cell activation via the T cell receptor (T3-Ti complex) by OKT3 results in modulation of the T3-Ti complex, but does not affect T4, T8, or T11 antigen expression. To study the effect of other T cell activators on these T cell membrane antigens, the authors incubated mononuclear cells for 0-3 days with lectins or pharmacologic agents and stained with monoclonal antibodies to their antigens. The median fluorescence intensity (MFI) was measured with a fluorescence activated cell sorter. Activation of PBL with Con A, PHA, calcium ionophore A23187, or with dbcAMP, isoproterenol, or theophyllin had minimal effects on the MFI of T3, T4, T8, or T11. Phorbol myristate acetate (PMA), a protein kinase C activator which stimulates PBL though an alternate pathway, caused a 90-100% reduction of T3 and T4 MFI, a 25% reduction in T8 MFI, and a 400% increase in T11 MFI after 2 days. Addition of A23187 slightly increased these effects. PMA induced a 2-3-fold increase in cell diameter concomitant with the alterations in membrane antigens. These data suggest that T cell activation through pathways not directly linked to the T cell antigen receptor can result in surface antigen expression different from that which follows activation via the T cell receptor.
- Research Organization:
- Univ. of Chicago, IL
- OSTI ID:
- 5499363
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LYMPHOCYTES
RECEPTORS
PHORBOL ESTERS
BIOLOGICAL EFFECTS
AMP
ANTIGENS
CELL MEMBRANES
CONCANAVALIN
FLUORESCENCE
LECTINS
MONOCLONAL ANTIBODIES
PHYTOHEMAGGLUTININ
AGGLUTININS
ANIMAL CELLS
ANTIBODIES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARCINOGENS
CELL CONSTITUENTS
CONNECTIVE TISSUE CELLS
ESTERS
HEMAGGLUTININS
LEUKOCYTES
LUMINESCENCE
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
MITOGENS
MUCOPROTEINS
NUCLEOTIDES
ORGANIC COMPOUNDS
POLYSACCHARIDES
PROTEINS
SACCHARIDES
SOMATIC CELLS
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)