Nephrotoxic effect of cadmium as cadmium chloride or cadmium metallothionein in renal cortical slices
The mechanism of cadmium-metallothinoein (CdMT) induced nephrotoxicity was studied in isolated rat kidney cortical slices. Accumulation of the organic anion, p-aminohippuric acid (PAH) and the organic cation, tetraethyl ammonium (TEA) into renal slices (0.5 mm) was measured after in vitro exposure of the slices with different concentrations of Cd/sup 2 +/ (5 x 10/sup -6/ to 2 x 10/sup -4/) as either CdCl/sub 2/ or CdMT for 90 minutes at 25/sup 0/C under 100% O/sub 2/. The concentrations of Cd, PAH and TEA were measured in the slices and media after incubation. The uptake of TEA was not significantly altered in the presence of either CdCl/sub 2/ or CdMT while PAH uptake by the slices was inhibited by both Cd compounds. The inhibitory effect of PAH uptake was dose dependent with both Cd compounds, however CdMT caused a greater effect at low doses as compared to CdCl/sub 2/. This may be due to a preferential uptake of Cd as CdMT by the slices. The intracellular presence of zinc metallothionein (ZnMT) or the in vitro addition of zinc salts may affect the toxic effect of these Cd compounds. The results show that the inhibitory effects of the Cd compounds were specific for the anionic transport in renal slices.
- Research Organization:
- Univ. of Western Ontario, London
- OSTI ID:
- 5499185
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
AMMONIUM COMPOUNDS
UPTAKE
CADMIUM
TOXICITY
HIPPURIC ACID
ANIONS
CATIONS
DOSE-RESPONSE RELATIONSHIPS
IN VITRO
KIDNEYS
METALLOTHIONEIN
RATS
AMINO ACIDS
ANIMALS
BODY
CARBOXYLIC ACIDS
CHARGED PARTICLES
ELEMENTS
IONS
MAMMALS
METALLOPROTEINS
METALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RODENTS
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)