Nephrotoxic effect of cadmium as cadmium chloride or cadmium metallothionein in renal cortical slices

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5499185

Suzuki, C A.M.; Cherian, M G

The mechanism of cadmium-metallothinoein (CdMT) induced nephrotoxicity was studied in isolated rat kidney cortical slices. Accumulation of the organic anion, p-aminohippuric acid (PAH) and the organic cation, tetraethyl ammonium (TEA) into renal slices (0.5 mm) was measured after in vitro exposure of the slices with different concentrations of Cd/sup 2 +/ (5 x 10/sup -6/ to 2 x 10/sup -4/) as either CdCl/sub 2/ or CdMT for 90 minutes at 25/sup 0/C under 100% O/sub 2/. The concentrations of Cd, PAH and TEA were measured in the slices and media after incubation. The uptake of TEA was not significantly altered in the presence of either CdCl/sub 2/ or CdMT while PAH uptake by the slices was inhibited by both Cd compounds. The inhibitory effect of PAH uptake was dose dependent with both Cd compounds, however CdMT caused a greater effect at low doses as compared to CdCl/sub 2/. This may be due to a preferential uptake of Cd as CdMT by the slices. The intracellular presence of zinc metallothionein (ZnMT) or the in vitro addition of zinc salts may affect the toxic effect of these Cd compounds. The results show that the inhibitory effects of the Cd compounds were specific for the anionic transport in renal slices.

• Research Organization: Univ. of Western Ontario, London
• OSTI ID: 5499185
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
AMMONIUM COMPOUNDS
ANIMALS
ANIONS
BODY
CADMIUM
CARBOXYLIC ACIDS
CATIONS
CHARGED PARTICLES
DOSE-RESPONSE RELATIONSHIPS
ELEMENTS
HIPPURIC ACID
IN VITRO
IONS
KIDNEYS
MAMMALS
METALLOPROTEINS
METALLOTHIONEIN
METALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RATS
RODENTS
TOXICITY
UPTAKE
VERTEBRATES