Cholinergic stimulation of phosphoinositide hydrolysis in rabbit kidney slices

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5497173

Garg, L C; McArdle, S; Crews, F T

The release of inositol phosphates (IP) from phosphoinositides (PI) by carbachol was studied in the tissue slices from cortex (C), outer medulla (OM) and inner medulla (IM) of rabbit kidneys. The method involved the incubation of the slices with (/sup 3/H)inositol for its incorporation into the PI and measurement of the release of IP in presence of lithium which prevents dephosphorylation of IP. The results of (/sup 3/H)IP formation are expressed as % of total (/sup 3/H)inositol incorporation in the tissue. No significant effect of carbachol was found on the release of IP in the C. The drug produced a 48% increase in IP release in the OM. In the IM, carbachol produced a concentration dependent increase in IP release with a maximum of 772% at 1 mM. The release of IP in the IM by 1 mM carbachol was completely blocked by 1 ..mu..M atropine. Our results indicate that IP release by carbachol is due to activation of muscarinic receptors in the IM of the rabbit kidney.

• Research Organization: Univ. of Florida, Gainesville
• OSTI ID: 5497173
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BODY
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
DRUGS
HYDROLYSIS
INOSITOL
INOSITOLS
ISOTOPE APPLICATIONS
KIDNEYS
KINETICS
LABELLED COMPOUNDS
LYSIS
MAMMALS
MEMBRANE PROTEINS
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PARASYMPATHOMIMETICS
PHOSPHATES
PHOSPHORUS COMPOUNDS
PROTEINS
RABBITS
REACTION KINETICS
RECEPTORS
SACCHARIDES
SOLVOLYSIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES