Intracellular calcium rise is not a necessary step for the stimulated actin polymerization

Conference · Sat Mar 01 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5494294

Yassin, R

Stimulation of rabbit peritoneal neutrophils by many chemotactic (formyl Methionyl-Leucyl-Phenylalanine (fMLP), Leukotriene B/sub 4/ (LTB/sub 4/)) and non-chemotactic (phorbol 12-myristate, 13-acetate (PMA), platelet activating factor (PAF), and the calcium ionophore A23187) factors produces rapid and dose dependent increases in the amount of actin associated with the cytoskeleton. The stimulated increase in cytoskeletal actin does not appear to require a rise in the intracellular concentration of free calcium. The increase in cytoskeletal actin produced by A23187 is transient and does not depend on the presence of calcium in the suspending medium. In the presence of extracellular calcium, the effect of the ionophore is biphasic with respect to concentration. The increases in actin association with cytoskeletal produced by fMLP, LTB/sub 4/, and A23187 but not by PMA, are inhibited by hyperosmolarity and pertussis toxin pretreatment. On the other hand, the addition of hyperosmolarity or pertussis toxin has small effect on the rise in the intracellular calcium produced by A23187. The results presented here suggest that an increase in the intracellular concentration of free calcium is not necessary for the stimulated increases in cytoskeletal actin.

• Research Organization: Univ. of Connecticut Health Center, Farmington
• OSTI ID: 5494294
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACTIN
SECRETION
BLOOD COAGULATION FACTORS
BIOLOGICAL EFFECTS
CALCIUM
PHENYLALANINE
PHORBOL ESTERS
DOSE-RESPONSE RELATIONSHIPS
NEUTROPHILS
RABBITS
ALKALINE EARTH METALS
AMINO ACIDS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CARCINOGENS
COAGULANTS
DRUGS
ELEMENTS
ESTERS
HEMATOLOGIC AGENTS
LEUKOCYTES
MAMMALS
MATERIALS
METALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)