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Drastic G/sub 2/ arrest in mammalian cells after irradiation with heavy-ion beams. [/sup 40/Ar, /sup 12/C, /sup 20/Ne beams; V79 cultured cells]

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3575025· OSTI ID:5487668
Unsynchronized exponentially growing V79 monolayer cultures were exposed to high-energy accelerated heavy-ion beams (/sup 12/C, /sup 20/Ne, /sup 40/Ar) at various positions in their unmodified Bragg ionization curves. High-LET particle beams proved to be much more effective in causing a G/sub 2/ + M block, measured by the method of flow microfluorometry, than low-LET radiation. The number of cells arrested in G/sub 2/ + M increased linearly with dose up to at least 75% of the maximum effect. In this domain, the RBE values were LET-dependent with a maximum (RBE/sub 50/ = 8.3) at about 100 keV/..mu..m. In contrast to ..gamma.. and x rays, heavy ions with high LET did not affect cell traverse through the G/sub 1/ and S phase. Though the RBE values for G/sub 2/ + M block are much higher than the RBEs for survival, there was a linear relationship between accumulation of cells in G/sub 2/ + M and cell killing.
Research Organization:
Univ. of California, Berkeley
OSTI ID:
5487668
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 19:1; ISSN RAREA
Country of Publication:
United States
Language:
English