Inactivation of synchronized Chinese hamster V79 cells with charged-particle track segments

Journal Article · Thu May 01 04:00:00 EDT 1980 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575379· OSTI ID:5129518

Bird, R P ^[1]; Rohrig, N; Colvett, R D; Geard, C R; Marino, S A

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1. Columbia Univ., New York, NY

Synchronized Chinese hamster V79 cells were irradiated with protons, deuterons, and helium-3 ions in a series of cell inactivation experiments. The charged particles were accelerated at the Radiological Research Accelerator Facility at Brookhaven National Laboratory and provided a range of LET values from 10 to 170 keV/..mu..m. Two parts of the cell cycle were irradiated to determine clonogenic survival, the G/sub 1//S transition and late-S phase, both obtained by hydroxyurea-induced synchrony. The difference in radiation sensitivity between these two synchronized cell populations decreased with increased values of LET, but a 10-fold difference persisted with the highest LET values. The inactivation cross sections calculated from initial slopes of the survival curves increased to plateau values of about 19 and 38 ..mu..m/sup 2/ for the late-S and G/sub 1//S cells, respectively. In contrast, the respective cross sections of cell nuclei were measured and had mean values of 222 and 165 ..mu..m/sup 2/.

• DOE Contract Number: EY-76-C-02-0016
• OSTI ID: 5129518
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 82:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BARYONS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CULTURES
CELL CYCLE
CELL KILLING
CELL PROLIFERATION
CHARGED PARTICLES
DATA
DEUTERONS
ELEMENTARY PARTICLES
ENERGY TRANSFER
EVEN-ODD NUCLEI
EXPERIMENTAL DATA
FERMIONS
HADRONS
HAMSTERS
HELIUM 3
HELIUM ISOTOPES
INFORMATION
ISOTOPES
LET
LIGHT NUCLEI
MAMMALS
NUCLEI
NUCLEONS
NUMERICAL DATA
PROTONS
RADIATION EFFECTS
RADIOSENSITIVITY
RODENTS
STABLE ISOTOPES
SURVIVAL CURVES
SYNCHRONOUS CULTURES
VERTEBRATES