Theory of optimization of the experimental conditions of preparative elution using the ideal model of liquid chromatography

Journal Article · Thu Jun 01 04:00:00 EDT 1989 · Analytical Chemistry (Washington); (United States)

DOI:https://doi.org/10.1021/ac00186a020· OSTI ID:5479161

Golshan-Shirazi, Sadroddin; Guiochon, G ^[1]

---

1. Univ. of Tennessee, Knoxville (USA) Oak Ridge National Lab., TN (USA)

Using the ideal model of chromatography, the authors have derived analytical expressions for the purity, the recovery yield, and the production rate in overloaded elution chromatography, in the case of a binary mixture, when the equilibrium isotherm of the components of the studied mixture can be described properly by the competitive Langmuir equations. The effects of the phase selectivity, the composition of the feed, the sample size, and the required degree of purity of the purified fractions are discussed. The optimum conditions are very different depending on whether one is more interested in the first or the second component of the mixture. In both cases, the production rate increases very rapidly with the selectivity of the phase system (almost as ({alpha} {minus} 1){sup 2} for low {alpha} values). The production rate for the first component increases with increasing sample size up to very large sample loads; the optimum depends on the lowest acceptable value of the yield. For the second component there is an optimum sample size which can be calculated easily, using a very simple equation, and which remains practically unchanged for real columns with finite efficiencies. If sample sizes larger than this optimum value are used, the production rate remains constant or decreases while the recovery yield drops.

• OSTI ID: 5479161
• Journal Information: Analytical Chemistry (Washington); (United States), Journal Name: Analytical Chemistry (Washington); (United States) Vol. 61:11; ISSN ANCHA; ISSN 0003-2700
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400105* -- Separation Procedures
ANALYTICAL SOLUTION
BINARY MIXTURES
CALCULATION METHODS
CHROMATOGRAPHY
DISPERSIONS
EXTRACTION APPARATUSES
EXTRACTION COLUMNS
LIQUID COLUMN CHROMATOGRAPHY
MIXTURES
OPTIMIZATION
SEPARATION EQUIPMENT
SEPARATION PROCESSES