Nickel in plants. I. Uptake kinetics using intact soybean seedlings

Journal Article · Sun Jan 01 04:00:00 EST 1978 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.62.4.563· OSTI ID:5478766

Cataldo, D A; Garland, T R; Wildung, R E

The absorption of Ni/sup 2 +/ by 21-day-old soybean plants (Glycine max. cv. Williams) was investigated with respect to its concentration dependence, transport kinetics, and interactions with various nutrient cations. Nickel absorption, measured as a function of concentration (0.02 to 100 ..mu..M), demonstrated the presence of multiple absorption isotherms. Each of the three isotherms conforms to Michaelis-Menten kinetics; kinetic constants are reported for uptake by the intact plant and for transfer from root to shoot tissues. The absorption of Ni/sup 2 +/ by the intact plant and its transfer from root to shoot were inhibited by the presence of Cu/sup 2 +/, Zn/sup 2 +/, Fe/sup 2 +/, and Co/sup 2 +/. Competition kinetics studies showed Cu/sup 2 +/ and Zn/sup 2 +/ to inhibit Ni/sup 2 +/ absorption competitively, suggesting that Ni/sup 2 +/, Cu/sup 2 +/, and Zn/sup 2 +/ are absorbed using the same carrier site. Calculated K/sub m/ and K/sub i/ constants for Ni/sup 2 +/ in the presence and absence of Cu/sup 2 +/ were 6.1 and 9.2 ..mu..m, respectively, whereas K/sub m/ and K/sub i/ constants were calculated to be 6.7 and 24.4 ..mu..M, respectively, for Ni/sup 2 +/ in the presence and absence of Zn/sup 2 +/. The mechanism of inhibition of Ni/sup 2 +/ in the presence of Fe/sup 2 +/ and Co/sup 2 +/ was not resolved by classical kinetic relationships. 13 references, 3 figures, 2 tables.

• Research Organization: Battelle, Pacific Northwest Labs., Richland, WA
• OSTI ID: 5478766
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 62:4; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

560303* -- Chemicals Metabolism & Toxicology-- Plants-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ABSORPTION
BIOCHEMICAL REACTION KINETICS
CATIONS
CHARGED PARTICLES
COBALT COMPOUNDS
COPPER COMPOUNDS
ELEMENTS
GLYCINE HISPIDA
IONS
IRON COMPOUNDS
ISOTHERMS
KINETICS
LEGUMINOSAE
MEMBRANE TRANSPORT
METALS
NICKEL
NUTRIENTS
PLANTS
REACTION KINETICS
RESPONSE MODIFYING FACTORS
ROOT ABSORPTION
ROOTS
SEEDLINGS
TRANSITION ELEMENT COMPOUNDS
TRANSITION ELEMENTS
TRANSLOCATION
UPTAKE
ZINC COMPOUNDS