Inhibition of glycogen synthase (casein) kinase-1 by different divalent metal ions
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6065413
The specificity of glycogen synthase (casein) kinase-1 (CK-1) for different divalent metal ions was explored in this study. Of the eight metal ions (Mg, Mn, Zn, Cu, Ca, Ni, Co, Fe) tested only Mg/sup 2 +/ supported significant kinase activity. Several of the other metals, however, inhibited the Mg/sup 2 +/-stimulated kinase activity. Half-maximal inhibition by Zn, Mn, Co, Fe and Ni were observed at 50, 55, 110, 125 and 300 ..mu..M, respectively. Kinetic analyses using Zn/sup 2 +/ indicate that at a constant (ATP) Zn/sup 2 +/ increases the K/sub m/ for casein, the V/sub max/ being unaffected +/- Zn/sup 2 +/. At a constant (casein), Zn/sup 2 +/ decreased the V/sub max/ for ATP, the K/sub m/ remaining unchanged +/- Zn/sup 2 +/. The inhibition of CK-1 by the different metals is reversed by EGTA or EDTA. These results suggest that divalent metal ions such as Zn/sup 2 +/ and Mn/sup 2 +/ that do not support CK-1 activity may serve as inhibitors of the kinase in vivo.
- Research Organization:
- Univ. of Waterloo, Ontario
- OSTI ID:
- 6065413
- Report Number(s):
- CONF-870644-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ALKALINE EARTH METAL COMPOUNDS
AMINO ACIDS
BIOLOGICAL EFFECTS
CALCIUM COMPOUNDS
CARBOXYLIC ACIDS
CATIONS
CHARGED PARTICLES
CHELATING AGENTS
COBALT COMPOUNDS
COPPER COMPOUNDS
EDTA
EGTA
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
GLYCOLS
HYDROXY COMPOUNDS
INHIBITION
IONS
IRON COMPOUNDS
MAGNESIUM COMPOUNDS
MANGANESE COMPOUNDS
NICKEL COMPOUNDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
TRANSFERASES
TRANSITION ELEMENT COMPOUNDS
ZINC COMPOUNDS
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ALKALINE EARTH METAL COMPOUNDS
AMINO ACIDS
BIOLOGICAL EFFECTS
CALCIUM COMPOUNDS
CARBOXYLIC ACIDS
CATIONS
CHARGED PARTICLES
CHELATING AGENTS
COBALT COMPOUNDS
COPPER COMPOUNDS
EDTA
EGTA
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
GLYCOLS
HYDROXY COMPOUNDS
INHIBITION
IONS
IRON COMPOUNDS
MAGNESIUM COMPOUNDS
MANGANESE COMPOUNDS
NICKEL COMPOUNDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
TRANSFERASES
TRANSITION ELEMENT COMPOUNDS
ZINC COMPOUNDS