GnRH receptors in human granulosa cells: Anatomical localization and characterization by autoradiographic study
Journal Article
·
· Endocrinology; (USA)
- Institut Pasteur, Paris (France)
The presence of receptors for GnRH in human ovary has been investigated by quantitative autoradiography. Simultaneous visualization and characterization of specific receptors on frozen sections were obtained on six pairs of human ovaries. Among them only one exhibited a large preovulatory follicle. This dominant follicle exhibited a specific and high affinity binding capacity for {sup 125}I-GnRHa exclusively localized on the granulosa cell layer. Analysis of saturation curve indicates a Kd value of 0.22 nM and Bmax of 9.6 fmol/mg protein. In contrast LH-hCG binding sites were present in all antral follicles. These data demonstrate for the first time the presence of high affinity GnRH receptors in human granulosa cells at a late stage of follicular maturation.
- OSTI ID:
- 5478759
- Journal Information:
- Endocrinology; (USA), Journal Name: Endocrinology; (USA) Vol. 125:3; ISSN ENDOA; ISSN 0013-7227
- Country of Publication:
- United States
- Language:
- English
Similar Records
Evidence for selective expression of angiotensin II receptors on atretic follicles in the rat ovary: an autoradiographic study
Localization, characterization, and quantification of insulin-like growth factor-I-binding sites in the ewe ovary
Characterization of angiotensin I-converting enzyme (ACE)-containing follicles in the rat ovary during the estrous cycle and effects of ACE inhibitor on ovulation
Journal Article
·
Wed Jun 01 00:00:00 EDT 1988
· Endocrinology; (United States)
·
OSTI ID:6924742
Localization, characterization, and quantification of insulin-like growth factor-I-binding sites in the ewe ovary
Journal Article
·
Tue Oct 31 23:00:00 EST 1989
· Endocrinology; (USA)
·
OSTI ID:5026572
Characterization of angiotensin I-converting enzyme (ACE)-containing follicles in the rat ovary during the estrous cycle and effects of ACE inhibitor on ovulation
Journal Article
·
Fri Jun 01 00:00:00 EDT 1990
· Endocrinology; (USA)
·
OSTI ID:6699326
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
BODY
CHEMICAL COMPOSITION
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
FEMALE GENITALS
GONADS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
MAMMALS
MAN
MEMBRANE PROTEINS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
OVARIES
PRIMATES
PROTEINS
RADIOISOTOPES
RECEPTORS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
BODY
CHEMICAL COMPOSITION
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
FEMALE GENITALS
GONADS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
MAMMALS
MAN
MEMBRANE PROTEINS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
OVARIES
PRIMATES
PROTEINS
RADIOISOTOPES
RECEPTORS
VERTEBRATES