Evidence for selective expression of angiotensin II receptors on atretic follicles in the rat ovary: an autoradiographic study
Ovarian angiotensin II (Ang II) receptors display a cyclical pattern of variation during the rat estrous cycle. Ang II receptors, estimated by the specific binding of the Ang II receptor antagonist (/sup 125/I)iodo-(Sar1,Ile8) Ang II to ovarian membranes, were lowest at estrus (binding site density (Bmax) = 35 +/- 2 fmol/mg; binding site affinity (KD) = 2.0 +/- 0.2 nM) and highest at diestrus I (Bmax = 59 +/- 3 fmol/mg; KD = 1.6 +/- 0.1 nM). We have previously shown that Ang II receptors in the rat ovary predominantly exist on the granulosa cell layer of a subpopulation of follicles. Our present studies show that the Ang II receptor-containing follicles in the rat ovary are mainly atretic (approximately 80%) or show signs of early atresia (approximately 15%) during all stages of the estrous cycle. A small number of Ang II receptor-containing follicles were healthy (approximately 5%). In contrast to the Ang II receptor-containing follicles, the FSH receptor-containing follicles were predominantly healthy (greater than 90%). Follicles which contained both Ang II receptors and FSH receptors were mainly early atretic. Since Ang II receptor-containing follicles in the rat ovary were mainly atretic these studies suggest that in the rat Ang II may be a major factor in regulating the function of atretic ovarian follicles.
- Research Organization:
- Cleveland Clinic Foundation, OH (USA)
- OSTI ID:
- 6924742
- Journal Information:
- Endocrinology; (United States), Vol. 122:6
- Country of Publication:
- United States
- Language:
- English
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AUTORADIOGRAPHY
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ESTROUS CYCLE
IODINE 125
RATS
TISSUE DISTRIBUTION
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
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CELL CONSTITUENTS
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DRUGS
ELECTRON CAPTURE RADIOISOTOPES
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INTERMEDIATE MASS NUCLEI
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ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
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550201* - Biochemistry- Tracer Techniques