Physical linkage of a human immunoglobulin heavy chain variable region gene segment to diversity and joining region elements

Journal Article · Tue Nov 01 04:00:00 EST 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.21.8196· OSTI ID:5478447

Schroeder, Jr, H W; Walter, M A; Hofker, M H; Ebens, A; Van Dijk, K W; Liao, L C; Cox, D W; Milner, E C.B.; Perlmutter, R M ^[1]

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1. Univ. of Washington, Seattle (USA)

Antibody genes are assembled from a series of germ-line gene segments that are juxtaposed during the maturation of B lymphocytes. Although diversification of the adult antibody repertoire results in large part from the combinatorial joining of these gene segments, a restricted set of antibody heavy chain variable (V{sub H}), diversity (D{sub H}), and joining (J{sub H}) region gene segments appears preferentially in the human fetal repertoire. The authors report here that one of these early-expressed V{sub H} elements (termed V{sub H}6) is the most 3' V{sub H} gene segment, positioned 77 kilobases on the 5' side of the J{sub H} locus and immediately adjacent to a set of previously described D{sub H} sequences. In addition to providing a physical map linking human V{sub H}, D{sub H}, and J{sub H} elements, these results support the view that the programmed development of the antibody V{sub H} repertoire is determined in part by the chromosomal position of these gene segments.

• OSTI ID: 5478447
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 85:21; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMAL CELLS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DNA SEQUENCING
ELECTROPHORESIS
GENES
GENETIC MAPPING
GLOBULINS
IMMUNOGLOBULINS
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
LYMPHOCYTES
MAPPING
MATERIALS
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION