X-ray microanalysis of electrolyte content of normal, preneoplastic, and neoplastic mouse mammary tissue

Journal Article · Thu Jan 01 04:00:00 EST 1981 · Cancer Res.; (United States)

OSTI ID:5478412

Smith, N K; Stabler, S B; Cameron, I L; Medina, D

Intracellular sodium, chlorine, and potassium concentrations (mmol/kg dry weight) were determined by electron probe X-ray microanalysis of individual epithelial cells in freeze-dried 2-micrometer sections of mouse mammary tissue which were cut at -30 degrees. A model system was utilized in order to compare elemental content of cells from normal pregnant mammary tissue and preneoplastic and neoplastic mammary tissues from female BALB/cCrlMed mice. Animals were killed by cervical dislocation, and tissue was rapidly frozen in liquid propane. Normal mammary glands were obtained from primiparous mice at 16 to 17 days of gestation. Tissue from the hyperplastic alveolar nodule line D1 was removed from donor mice 12 to 16 weeks after transplantation into the cleared mammary fat pad. All mammary adenocarcinomas, D1T, were primary tumors which developed in mice with transplants of nodule line D1. Data were collected from five animals (10 cells/animal) in each of the three groups. It was found that the electrolyte content of cells of preneoplastic tissue was the same as that of the normal mammary tissue but was significantly elevated in neoplastic tissue (162, 130, and 48% increases for sodium, chlorine, and potassium, respectively). Thus, an increase in electrolyte content seems to be associated with the transformation to a neoplastic state and not associated with conversion to the preneoplastic state.

• Research Organization: Department of Anatomy, The University of Texas Health Science Center at San Antonio
• OSTI ID: 5478412
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 41:10; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

550602 -- Medicine-- External Radiation in Diagnostics-- (1980-)
550900* -- Pathology
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ALKALI METAL COMPOUNDS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANIONS
BODY
CARCINOMAS
CATIONS
CHARGED PARTICLES
CHEMICAL ANALYSIS
CHLORINE
DIAGNOSIS
DISEASES
ELECTROLYTES
ELECTROMAGNETIC RADIATION
ELECTRON MICROPROBE ANALYSIS
ELEMENTS
EPITHELIUM
GLANDS
HALOGENS
IONIZING RADIATIONS
IONS
MAMMALS
MAMMARY GLANDS
MICE
MICROANALYSIS
NEOPLASMS
NONMETALS
ONCOGENIC TRANSFORMATIONS
ORGANS
PATHOLOGY
POTASSIUM COMPOUNDS
RADIATIONS
RODENTS
SODIUM COMPOUNDS
TISSUES
VERTEBRATES
X RADIATION