Effect of oxygen deprivation on metabolism of arachidonic acid by cultures of rat heart cells

Journal Article · Tue Aug 01 04:00:00 EDT 1989 · American Journal of Physiology; (USA)

OSTI ID:5477384

Freyss-Beguin, M; Millanvoye-van Brussel, E; Duval, D ^[1]

---

1. Hopital Necker, Paris (France)

To investigate the mechanisms responsible for the impairment of phospholipid metabolism observed in ischemic cells, we have studied the effect of conditions simulating ischemia on the metabolism of arachidonic acid (AA) by muscle (M-) and nonmuscle (F-) cells isolated from newborn rat hearts and cultured separately. In muscle cells, oxygen deprivation induces a significant stimulation of the release of ({sup 14}C)AA from prelabeled cells associated with a preferential redistribution of ({sup 14}C)AA into cell triglycerides but not formation of radioactive prostaglandins. Moreover, the fatty acid content of phospholipids, as measured by capillary gas chromatography, appears markedly reduced in ischemic myocardial cells. This fact may be related to phospholipase stimulation during ischemia as suggested by the antagonistic effect of mepacrine or p-bromophenacyl bromide. In contrast, oxygen deprivation failed to induce any significant alteration of AA metabolism in fibroblast-like heart cells. Our results indicate that these cultures of newborn rat heart cells, which exhibit many of the features observed in intact organ during ischemia, may represent a useful experimental model to investigate the pharmacological control of the membrane phospholipid turnover.

• OSTI ID: 5477384
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 257; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANOXIA
ARACHIDONIC ACID
BIOLOGICAL EFFECTS
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CELL CULTURES
CHROMATOGRAPHY
DISEASES
ESTERS
GAS CHROMATOGRAPHY
HEART
ISCHEMIA
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
MAMMALS
METABOLISM
MONOCARBOXYLIC ACIDS
MUSCLES
MYOCARDIUM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PATHOGENESIS
PHOSPHOLIPIDS
RATS
RODENTS
SEPARATION PROCESSES
THIN-LAYER CHROMATOGRAPHY
TRACER TECHNIQUES
TRIGLYCERIDES
VASCULAR DISEASES
VERTEBRATES