Biochemical and subcellular distribution of arachidonic acid in rat myocardium

Journal Article · Tue Dec 01 04:00:00 EST 1987 · American Journal of Physiology; (USA)

OSTI ID:7191815

Miyazaki, Y; Gross, R W; Sobel, B E; Saffitz, J E ^[1]

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1. Washington Univ., St. Louis, MO (USA)

Selective release of arachidonic acid from prelabeled phospholipid pools has been observed following exposure of neonatal rat cardiac myocytes to metabolic inhibitors in vitro and has been correlated temporally with the development of irreversible sarcolemmal damage. Hydrolysis of phospholipids with release of arachidonic acid may be an important mechanism in the pathogenesis of sarcolemmal damage induced by ischemia. To elucidate potential subcellular loci of arachidonic acid release in ischemic myocardium, the authors characterized the phospholipid composition of adult rat myocardial sarcolemma and delineated the biochemical and subcellular distribution of radiolabeled arachidonic acid in neonatal rat myocytes incubated with ({sup 3}H)-arachidonic acid for selected intervals. Radioactivity was located almost exclusively in mitochondria and internal cytoplasmic membranes (primarily sarcoplasmic reticulum), which collectively contained 90% of myocyte radioactivity. These results indicate that radiolabeled arachidonic acid released from prelabeled phospholipid pools on exposure of neonatal rat myocytes to oxidative inhibitors is derived from mitochondria and internal cell membranes. The diminutive labeling of the sarcolemma suggests that turnover of arachidonoyl phospholipids is slower in the sarcolemma than in other membranous organelles.

• OSTI ID: 7191815
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 253:6; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ARACHIDONIC ACID
AUTORADIOGRAPHY
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
CELL CULTURES
CHEMICAL REACTIONS
DECOMPOSITION
DISEASES
DISTRIBUTION
ELECTRON MICROSCOPY
ESTERS
HEART
HYDROGEN COMPOUNDS
HYDROLYSIS
ISCHEMIA
LIPIDS
LYSIS
MAMMALS
MICROSCOPY
MONOCARBOXYLIC ACIDS
MUSCLES
MYOCARDIUM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHOLIPIDS
RATS
RODENTS
SOLVOLYSIS
SUBCELLULAR DISTRIBUTION
TRANSMISSION ELECTRON MICROSCOPY
TRITIUM COMPOUNDS
VASCULAR DISEASES
VERTEBRATES